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Wheat Rust Diseases

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Sambasivam Periyannan

    1. Research School of Biology, Australian National University, Canberra, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1659)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Wheat Rust Surveillance and Genotyping

    1. Front Matter

      Pages 1-1
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    2. Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping

      • Sajid Ali, David Hodson
      Pages 3-11

    3. Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance

      • Vanessa Bueno-Sancho, Daniel C. E. Bunting, Luis J. Yanes, Kentaro Yoshida, Diane G. O. Saunders
      Pages 13-28

    4. Race Typing of Puccinia striiformis on Wheat

      • Mogens S. Hovmøller, Julian Rodriguez-Algaba, Tine Thach, Chris K. Sørensen
      Pages 29-40

    5. Assessment of Aggressiveness of Puccinia striiformis on Wheat

      • Chris K. Sørensen, Tine Thach, Mogens S. Hovmøller
      Pages 41-47

    6. Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing

      • Benjamin Schwessinger, John P. Rathjen
      Pages 49-57

    7. Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis

      • Sajid Ali, Muhammad R. Khan, Angelique Gautier, Zahoor A. Swati, Stephanie Walter
      Pages 59-70

  3. Molecular Pathogenicity

    1. Front Matter

      Pages 71-71
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    2. Computational Methods for Predicting Effectors in Rust Pathogens

      • Jana Sperschneider, Peter N. Dodds, Jennifer M. Taylor, Sébastien Duplessis
      Pages 73-83

    3. Protein–Protein Interaction Assays with Effector–GFP Fusions in Nicotiana benthamiana

      • Benjamin Petre, Joe Win, Frank L. H. Menke, Sophien Kamoun
      Pages 85-98

    4. Proteome Profiling by 2D–Liquid Chromatography Method for Wheat–Rust Interaction

      • Semra Hasançebi
      Pages 99-113

    5. Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing

      • Vinay Panwar, Guus Bakkeren
      Pages 115-124

    6. Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi

      • Veronica Roman-Reyna, John P. Rathjen
      Pages 125-134

  4. Genetics of Rust Resistance

    1. Front Matter

      Pages 135-135
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    2. Genetic Analysis of Resistance to Wheat Rusts

      • Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, Ravi P. Singh
      Pages 137-149

    3. Advances in Identification and Mapping of Rust Resistance Genes in Wheat

      • Urmil Bansal, Harbans Bariana
      Pages 151-162

    4. Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives

      • Peng Zhang, Ian S. Dundas, Steven S. Xu, Bernd Friebe, Robert A. McIntosh, W. John Raupp
      Pages 163-172

    5. Applications of Genomic Selection in Breeding Wheat for Rust Resistance

      • Leonardo Ornella, Juan Manuel González-Camacho, Susanne Dreisigacker, Jose Crossa
      Pages 173-182

    6. Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown under Controlled Conditions

      • Adnan Riaz, Lee T. Hickey
      Pages 183-196
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Keywords

About this book

This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.

Editors and Affiliations

  • Research School of Biology, Australian National University, Canberra, Australia

    Sambasivam Periyannan

Bibliographic Information

  • Book Title: Wheat Rust Diseases

  • Book Subtitle: Methods and Protocols

  • Editors: Sambasivam Periyannan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7249-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7248-7Published: 31 August 2017

  • Softcover ISBN: 978-1-4939-8430-5Published: 12 June 2018

  • eBook ISBN: 978-1-4939-7249-4Published: 30 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 294

  • Number of Illustrations: 17 b/w illustrations, 50 illustrations in colour

  • Topics: Plant Sciences

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