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The Bacterial Nucleoid

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Includes step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1624)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
  2. Molecular Genetic Methods to Study Bacterial Nucleoid

    1. Front Matter

      Pages 1-1
    2. Revealing Sister Chromatid Interactions with the loxP/Cre Recombination Assay

      • Elise Vickridge, Charlène Planchenault, Olivier Espéli
      Pages 29-37
    3. Transposon Insertion Site Sequencing for Synthetic Lethal Screening

      • Yoshiharu Yamaichi, Tobias Dörr
      Pages 39-49
  3. Study of Bacterial Nucleoid with Whole Genome Analysis Method

    1. Front Matter

      Pages 51-51
    2. WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites

      • Mickaël Poidevin, Elisa Galli, Yoshiharu Yamaichi, François-Xavier Barre
      Pages 53-60
    3. High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing

      • Roxanne E. Diaz, Aurore Sanchez, Véronique Anton Le Berre, Jean-Yves Bouet
      Pages 61-73
    4. Generation and Analysis of Chromosomal Contact Maps of Bacteria

      • Martial Marbouty, Romain Koszul
      Pages 75-84
    5. Nucleoid-Associated Proteins: Genome Level Occupancy and Expression Analysis

      • Parul Singh, Aswin Sai Narain Seshasayee
      Pages 85-97
  4. Molecular Biology Methods to Study Nucleoid Structuring Factors

    1. Front Matter

      Pages 99-99
    2. Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins

      • Sanja Mehandziska, Alexander M. Petrescu, Georgi Muskhelishvili
      Pages 101-116
    3. Tethered Particle Motion Analysis of the DNA Binding Properties of Architectural Proteins

      • Ramon A. van der Valk, Niels Laurens, Remus T. Dame
      Pages 127-143
    4. Biochemical Analysis of Bacterial Condensins

      • Zoya M. Petrushenko, Valentin V. Rybenkov
      Pages 145-159
    5. Exploring Condensins with Magnetic Tweezers

      • Rupa Sarkar, Valentin V. Rybenkov
      Pages 161-171
    6. Applications of Magnetic Tweezers to Studies of NAPs

      • Ricksen S. Winardhi, Jie Yan
      Pages 173-191
    7. A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data

      • Damien Larivière, Rodrigo Galindo-Murillo, Eric Fourmentin, Samuel Hornus, Bruno Lévy, Julie Papillon et al.
      Pages 193-210
  5. Imaging the Bacterial Nucleoid

    1. Front Matter

      Pages 211-211

About this book

This volume presents state-of-the-art protocols for key experiments that have revolutionized our understanding of the bacterial nucleoid. This book is divided into five parts: Part I introduces molecular genetic methods to study bacterial nucleoids; Part II highlights the study of bacterial nucleoid with whole genome analysis method; Part III discusses molecular biology methods to study nucleoid structuring factors; Part IV looks at imaging bacterial nucleoid; and Part V explores biophysics of the bacterial nucleoid. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Thorough and cutting-edge, The Bacterial Nucleoid: Methods and Protocols is a valuable resource that provides a wealth of new information about this chromosome.

Editors and Affiliations

  • UMR CNRS 7241, INSERM U1050, CIRB, Collège de France, Paris, France

    Olivier Espéli

Bibliographic Information

  • Book Title: The Bacterial Nucleoid

  • Book Subtitle: Methods and Protocols

  • Editors: Olivier Espéli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7098-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7097-1Published: 25 August 2017

  • Softcover ISBN: 978-1-4939-8384-1Published: 12 May 2018

  • eBook ISBN: 978-1-4939-7098-8Published: 25 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 374

  • Number of Illustrations: 74 b/w illustrations, 82 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access