Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1614)
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Table of contents (16 protocols)
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Front Matter
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Back Matter
About this book
This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that providesscientists and researchers with the best approaches to examine RAAS.
Editors and Affiliations
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Department of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, USA
Sean E. Thatcher
Bibliographic Information
Book Title: The Renin-Angiotensin-Aldosterone System
Book Subtitle: Methods and Protocols
Editors: Sean E. Thatcher
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7030-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7028-5Published: 13 May 2017
Softcover ISBN: 978-1-4939-8373-5Published: 08 August 2018
eBook ISBN: 978-1-4939-7030-8Published: 11 May 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 201
Number of Illustrations: 20 b/w illustrations, 38 illustrations in colour
Topics: Biochemistry, general