Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1605)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
About this book
This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA.
Editors and Affiliations
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Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, USA
Kiho Lee
Bibliographic Information
Book Title: Zygotic Genome Activation
Book Subtitle: Methods and Protocols
Editors: Kiho Lee
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6988-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6986-9Published: 29 April 2017
Softcover ISBN: 978-1-4939-8359-9Published: 09 June 2018
eBook ISBN: 978-1-4939-6988-3Published: 28 April 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 272
Number of Illustrations: 10 b/w illustrations, 41 illustrations in colour
Topics: Human Genetics