Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1597)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
About this book
This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.
Editors and Affiliations
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Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan
Takashi Tsuji
Bibliographic Information
Book Title: Organ Regeneration
Book Subtitle: 3D Stem Cell Culture & Manipulation
Editors: Takashi Tsuji
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6949-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6947-0Published: 31 March 2017
Softcover ISBN: 978-1-4939-8348-3Published: 09 June 2018
eBook ISBN: 978-1-4939-6949-4Published: 30 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 248
Number of Illustrations: 7 b/w illustrations, 60 illustrations in colour
Topics: Cell Biology