Skip to main content
SpringerLink
Log in
SpringerLink
Log in

Cell Cycle Synchronization

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Gaspar Banfalvi

    1. Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1524)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Cell Cycle Synchronization

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Overview of Cell Synchronization

      • Gaspar Banfalvi
      Pages 3-27

  3. Physical Fractionation

    1. Front Matter

      Pages 29-29
      Download chapter PDF

    2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

      • Gaspar Banfalvi
      Pages 31-52

    3. Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer Cells

      • Laura Senovilla, Yohann Demont, Juliette Humeau, Norma Bloy, Guido Kroemer
      Pages 53-64

    4. Large-Scale Mitotic Cell Synchronization

      • Kalyan Dulla, Anna Santamaria Margalef
      Pages 65-74

  4. Chemical Blockade

    1. Front Matter

      Pages 75-75
      Download chapter PDF

    2. Synchronization and Desynchronization of Cells by Interventions on the Spindle Assembly Checkpoint

      • Mohamed Jemaà, Gwenola Manic, Ilio Vitale
      Pages 77-95

    3. Synchronization of Mammalian Cell Cultures by Serum Deprivation

      • Thomas J. Langan, Kyla R. Rodgers, Richard C. Chou
      Pages 97-105

    4. DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibitors of DNA Replication: Analysis by Flow Cytometry

      • Dorota Halicka, Hong Zhao, Jiangwei Li, Jorge Garcia, Monika Podhorecka, Zbigniew Darzynkiewicz
      Pages 107-119

    5. Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias latipes

      • Takashi Iwamatsu
      Pages 121-147

    6. Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate

      • Cong-Jun Li
      Pages 149-159

    7. Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture

      • Gabor Nagy, Bence Tanczos, Eszter Fidrus, Laszlo Talas, Gaspar Banfalvi
      Pages 161-176

    8. Analysis of Nuclear Uracil DNA–Glycosylase (nUDG) Turnover During the Cell Cycle

      • Jennifer A. Fischer, Salvatore J. Caradonna
      Pages 177-188

    9. Synchronization of HeLa Cells

      • Hoi Tang Ma, Randy Y. C. Poon
      Pages 189-201

  5. Synchronization of Unicellular Organisms

    1. Front Matter

      Pages 203-203
      Download chapter PDF

    2. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

      • Gaspar Banfalvi
      Pages 205-213

    3. Synchronization of Yeast

      • Jessica Smith, Arkadi Manukyan, Hui Hua, Huzefa Dungrawala, Brandt L. Schneider
      Pages 215-242

    4. Synchronization of Pathogenic Protozoans

      • Staffan Svärd, Karin Troell
      Pages 243-252
Back to top

Keywords

About this book

This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second Edition cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.

Editors and Affiliations

  • Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary

    Gaspar Banfalvi

Bibliographic Information

  • Book Title: Cell Cycle Synchronization

  • Book Subtitle: Methods and Protocols

  • Editors: Gaspar Banfalvi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6603-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6602-8Published: 05 November 2016

  • Softcover ISBN: 978-1-4939-8248-6Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6603-5Published: 03 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 354

  • Number of Illustrations: 57 b/w illustrations, 14 illustrations in colour

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation