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Isolation of Plant Organelles and Structures

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Nicolas L. Taylor

    1. The University of Western Australia, Perth, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. A. Harvey Millar

    1. The University of Western Australia, Perth, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of plant organelles and structures
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1511)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Isolation of Plant Organelles and Structures in the Post-genomic Era

    • A. Harvey Millar, Nicolas L. Taylor
    Pages 1-11

  3. Approaches to Characterize Organelle, Compartment, or Structure Purity

    • Stefanie J. Mueller, Sebastian N. W. Hoernstein, Ralf Reski
    Pages 13-28

  4. Isolation of Organelles and Organelle Compartments

    1. Front Matter

      Pages 29-29
      Download chapter PDF

    2. Isolation of Nuclei and Nucleoli

      • Alison F. Pendle, Peter J. Shaw
      Pages 31-44

    3. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts

      • Úrsula Flores-Pérez, Paul Jarvis
      Pages 45-60

    4. Isolation of Chromoplasts and Suborganellar Compartments from Tomato and Bell Pepper Fruit

      • Cristina Barsan, Marcel Kuntz, Jean-Claude Pech
      Pages 61-71

    5. Leucoplast Isolation and Subfractionation

      • William C. Plaxton
      Pages 73-81

    6. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes

      • Owen Duncan, A. Harvey Millar, Nicolas L. Taylor
      Pages 83-96

    7. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane

      • Sigrun Reumann, Piotr Lisik
      Pages 97-112

    8. Isolation of Vacuoles and the Tonoplast

      • Jan Zouhar
      Pages 113-118

    9. Isolation of Endoplasmic Reticulum and Its Membrane

      • G. Eric Schaller
      Pages 119-129

    10. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings

      • Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, Joshua L. Heazlewood
      Pages 131-150

    11. Isolation of Autolysosomes from Tobacco BY-2 Cells

      • Chihiro Takatsuka, Yuko Inoue-Aono, Yuji Moriyasu
      Pages 151-161

    12. Isolation of Protein Storage Vacuoles and Their Membranes

      • Tomoo Shimada, Ikuko Hara-Nishimura
      Pages 163-168

  5. Isolation of Other Structures and Compartments

    1. Front Matter

      Pages 169-169
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    2. Isolation of the Cell Wall

      • Hervé Canut, Cécile Albenne, Elisabeth Jamet
      Pages 171-185

    3. Isolation of Plasmodesmata

      • Christine Faulkner, Emmanuelle M. F. Bayer
      Pages 187-198

    4. Isolation of Plasma Membrane and Plasma Membrane Microdomains

      • Anzu Minami, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura
      Pages 199-212

    5. Enrichment of the Plant Cytosolic Fraction

      • Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, Joshua L. Heazlewood
      Pages 213-232
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Keywords

About this book

This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.

Editors and Affiliations

  • The University of Western Australia, Perth, Australia

    Nicolas L. Taylor, A. Harvey Millar

Bibliographic Information

  • Book Title: Isolation of Plant Organelles and Structures

  • Book Subtitle: Methods and Protocols

  • Editors: Nicolas L. Taylor, A. Harvey Millar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6533-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6531-1Published: 12 October 2016

  • Softcover ISBN: 978-1-4939-8228-8Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6533-5Published: 11 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 338

  • Number of Illustrations: 22 b/w illustrations, 29 illustrations in colour

  • Topics: Plant Sciences

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