Receptor and Ion Channel Detection in the Brain

1. Front Matter

   Pages i-xviii

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2. Molecular Techniques

   1. Front Matter

      Pages 1-1

      PDF

   2. Production of High-Quality Antibodies for the Study of Receptors and Ion Channels

      • Masahiko Watanabe

      Pages 3-18

   3. Co-immunoprecipitation from Brain

      • Xavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe, Víctor Fernández-Dueñas, Francisco Ciruela

      Pages 19-29

   4. Subsynaptic Membrane Fractionation

      • Paula M. Canas, Rodrigo A. Cunha

      Pages 31-37

   5. Investigation of Neurotransmitter Receptors in Brain Slices Using Cell Surface Biotinylation

      • Elek Molnár

      Pages 39-48

   6. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells

      • Juan Varela, Julien Dupuis, Laurent Groc

      Pages 49-58

   7. Radioligand Binding Detection of Receptors in Brain Membranes

      • Fuencisla Pilar-Cuéllar, Alvaro Díaz, Emilio Garro-Martínez, Alicia Martín, Beatriz Romero, Elsa M. Valdizán

      Pages 59-76

   8. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain

      • Markus U. Ehrengruber, Kenneth Lundstrom

      Pages 77-97

   9. Fluorescent Ligands and TR-FRET to Study Receptor–Receptor Interactions in the Brain

      • Víctor Fernández-Dueñas, Thierry Durroux, Francisco Ciruela

      Pages 99-107

   10. In Situ Proximity Ligation Assay to Study and Understand the Distribution and Balance of GPCR Homo- and Heteroreceptor Complexes in the Brain

       • Dasiel O. Borroto-Escuela, Beth Hagman, Miles Woolfenden, Luca Pinton, Antonio Jiménez-Beristain, Julia Oflijan et al.

       Pages 109-124

3. Neuroanatomical Techniques

   1. Front Matter

      Pages 125-125

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   2. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling

      • Miwako Yamasaki, Masahiko Watanabe

      Pages 127-142

   3. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain

      • Rebeca Vidal, Raquel Linge, María Josefa Castillo, Josep Amigó, Elsa M. Valdizán, Elena Castro

      Pages 143-156

   4. Analysis of the Expression Profile and Regional Distribution of Neurotransmitter Receptors and Ion Channels in the Central Nervous System Using Histoblots

      • Elek Molnár

      Pages 157-170

   5. Immunohistochemistry for Ion Channels and Their Interacting Molecules: Tips for Improving Antibody Accessibility

      • Kohtarou Konno, Masahiko Watanabe

      Pages 171-178

   6. Localization of GFP-Tagged Proteins at the Electron Microscope

      • Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil et al.

      Pages 179-190

   7. Pre-embedding Methods for the Localization of Receptors and Ion Channels

      • Rafael Luján

      Pages 191-210

   8. Post-embedding Immunohistochemistry in the Localisation of Receptors and Ion Channels

      • Rafael Luján, Masahiko Watanabe

      Pages 211-232

   9. High-Resolution Localization of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)

      • Harumi Harada, Ryuichi Shigemoto

      Pages 233-245

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

• Biochemical approaches
• Electrophysiological recordings
• Fluorescence & FRET assays
• Neuroanatomical techniques
• Neurochemical determinations

• Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla- La Mancha, Albacete, Spain

  Rafael Luján

• Pharmacology Unit, Department of Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain

  Francisco Ciruela

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