Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1350)
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Table of contents (26 protocols)
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Front Matter
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Introduction
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Front Matter
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Baculovirus Molecular Biology/Development of Recombinant Baculoviruses
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Front Matter
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Protein Production with Recombinant Baculoviruses
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Front Matter
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About this book
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
Editors and Affiliations
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Engineering, University of Iowa Dept. Chemical & Biochemical, Iowa City, USA
David W. Murhammer
Bibliographic Information
Book Title: Baculovirus and Insect Cell Expression Protocols
Editors: David W. Murhammer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3043-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2016
Hardcover ISBN: 978-1-4939-3042-5Published: 29 January 2016
Softcover ISBN: 978-1-4939-7978-3Published: 31 March 2018
eBook ISBN: 978-1-4939-3043-2Published: 28 January 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XII, 508
Number of Illustrations: 50 b/w illustrations, 15 illustrations in colour
Topics: Microbiology, Virology