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Natural Products From Marine Algae

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Dagmar B. Stengel

    1. Botany and Plant Science, School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research,, National University of Ireland Galway, Galway, Ireland

  2. Solène Connan

    1. Photobiotechnology, INTECHMER, Conservatoire National des Arts et Métiers, Cherbourg, Cedex, France, CNRS, GEPEA, UMR6144, Boulevard de l’Université, Saint Nazaire, Cedex, France

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1308)

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About this book

This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments.

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Marine Algae: a Source of Biomass for Biotechnological Applications

    • Dagmar B. Stengel, Solène Connan
    Pages 1-37

  3. Structure and Function of Macroalgal Natural Products

    • Ryan M. Young, Kathryn M. Schoenrock, Jacqueline L. von Salm, Charles D. Amsler, Bill J. Baker
    Pages 39-73

  4. Spectrophotometric Assays of Major Compounds Extracted from Algae

    • Solène Connan
    Pages 75-101

  5. Extraction and Enrichment of Protein from Red and Green Macroalgae

    • Pádraigín A. Harnedy, Richard J. FitzGerald
    Pages 103-108

  6. Extraction and Purification of R-phycoerythrin from Marine Red Algae

    • Justine Dumay, Michèle Morançais, Huu Phuo Trang Nguyen, Joël Fleurence
    Pages 109-117

  7. Extraction and Analysis of Mycosporine-Like Amino Acids in Marine Algae

    • Nedeljka N. Rosic, Christoph Braun, David Kvaskoff
    Pages 119-129

  8. Extraction and Purification of Phlorotannins from Brown Algae

    • Erwan Ar Gall, Florian Lelchat, Mélanie Hupel, Camille Jégou, Valérie Stiger-Pouvreau
    Pages 131-143

  9. Enzyme-Enhanced Extraction of Antioxidant Ingredients from Algae

    • Björn V. Adalbjörnsson, Rósa Jónsdóttir
    Pages 145-150

  10. Microwave-Assisted Extraction of Fucoidan from Marine Algae

    • Solange I. Mussatto
    Pages 151-157

  11. Extraction and Analysis of Oxylipins from Macroalgae Illustrated on the Example Gracilaria vermiculophylla

    • Dominique Jacquemoud, Georg Pohnert
    Pages 159-172

  12. Lipids and Fatty Acids in Algae: Extraction, Fractionation into Lipid Classes, and Analysis by Gas Chromatography Coupled with Flame Ionization Detector (GC-FID)

    • Freddy Guihéneuf, Matthias Schmid, Dagmar B. Stengel
    Pages 173-190

  13. HRMAS NMR Analysis of Algae and Identification of Molecules of Interest via Conventional 1D and 2D NMR: Sample Preparation and Optimization of Experimental Conditions

    • Gaëlle Simon, Nelly Kervarec, Stéphane Cérantola
    Pages 191-205

  14. Extraction, Purification, and NMR Analysis of Terpenes from Brown Algae

    • Marc Gaysinski, Annick Ortalo-Magné, Olivier P. Thomas, Gérald Culioli
    Pages 207-223

  15. Extraction, Isolation, and Identification of Sesquiterpenes from Laurencia Species

    • Angélica Ribeiro Soares
    Pages 225-240

  16. The Use of HPLC for the Characterization of Phytoplankton Pigments

    • José L. Garrido, Suzanne Roy
    Pages 241-252

  17. Characterization of Phlorotannins from Brown Algae by LC-HRMS

    • Jeremy E. Melanson, Shawna L. MacKinnon
    Pages 253-266

  18. Analysis of Betaines from Marine Algae Using LC-MS-MS

    • Shawna L. MacKinnon, Cheryl Craft
    Pages 267-275

  19. Analysis of Marine Biotoxins Using LC-MS/MS

    • Bernd Luckas, Katrin Erler, Bernd Krock
    Pages 277-297

  20. Fucoidan Analysis by Tandem MALDI-TOF and ESI Mass Spectrometry

    • Stanislav D. Anastyuk, Natalia M. Shevchenko, Vladimir I. Gorbach
    Pages 299-312
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Editors and Affiliations

  • Botany and Plant Science, School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research,, National University of Ireland Galway, Galway, Ireland

    Dagmar B. Stengel

  • Photobiotechnology, INTECHMER, Conservatoire National des Arts et Métiers, Cherbourg, Cedex, France, CNRS, GEPEA, UMR6144, Boulevard de l’Université, Saint Nazaire, Cedex, France

    Solène Connan

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Bibliographic Information

  • Book Title: Natural Products From Marine Algae

  • Book Subtitle: Methods and Protocols

  • Editors: Dagmar B. Stengel, Solène Connan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2684-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2683-1Published: 25 June 2015

  • Softcover ISBN: 978-1-4939-5273-1Published: 13 October 2016

  • eBook ISBN: 978-1-4939-2684-8Published: 24 June 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 439

  • Number of Illustrations: 76 b/w illustrations, 34 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

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