Methods of Protein Separation

1. Front Matter

   Pages I-XIII

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2. Sedimentation and Gel-Permeation Chromatography of Associating-Dissociating Macromolecules

   • John R. Cann

   Pages 1-25

3. Trans Electrophoresis

   • Nicholas Catsimpoolas

   Pages 27-67

4. Immunodiffusion

   • Alfred J. Crowle

   Pages 69-92

5. Isoelectric Focusing in Polyacrylamide Gel

   • James W. Drysdale

   Pages 93-126

6. Purification of Chemically Modified Proteins

   • Robert E. Feeney, David T. Osuga

   Pages 127-160

7. Chromatographic Peak Shape Analysis

   • Eli Grushka

   Pages 161-192

8. Sedimentation Equilibrium of Proteins in Density Gradients

   • James B. Ifft

   Pages 193-237

9. Hollow-Fiber Separation Devices and Processes

   • Burton A. Zabin

   Pages 239-254

10. Affinity Chromatography, Principles and Applications

    • Indu Parikh, Pedro Cuatrecasas

    Pages 255-276

11. Back Matter

    Pages 277-281

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This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.

• amino acid
• biological
• biology
• development
• enzyme
• enzymes
• experiment
• ferritin
• nucleic acid
• protein
• proteins
• society
• tissue
• transport

• Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, USA

  Nicholas Catsimpoolas

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