Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions

1. Front Matter

   Pages N2-XVI

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2. Background of the Immune System

   1. Front Matter

      Pages 1-1

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   2. Understanding The Immune System

      • Office of Research Reporting and Public Response National Institute of Allergy and Infectious Diseases National Institutes of Health

      Pages 2-21

3. Background

   1. Front Matter

      Pages 23-23

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   2. What Does the Immunological Approach Offer Aquatic Research? An Overview

      • S. A. Pomponi, C. M. Yentsch, P. K. Horan

      Pages 24-40

   3. Molecular Biology, Single Cell Analysis and Quantitative Genetics: New Evolutionary Genetic Approaches in Phytoplankton Ecology

      • A. Michelle Wood

      Pages 41-71

4. Techniques

   1. Front Matter

      Pages 73-73

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   2. The Production of Monospecific Antisera to Soft-Bottom Benthic Taxa

      • Eugene D. Gallagher, Peter A. Jumars, Gary L. Taghon

      Pages 74-98

   3. Preparation and use of Monospecific Antisera to Capitella SP. I.

      • Eugene D. Gallagher, J. P. Grassle, D. D. Trueblood, M. G. Gleason

      Pages 99-99

   4. Cell Affinity Chromatography for a Marine Nitrifying Bacterium

      • U. Heyman, B. Heyman, Bess B. Ward

      Pages 100-116

   5. Application of Immunoblotting for Dietary Analysis

      • Gregory Zagursky, Robert J. Feller

      Pages 117-129

   6. Two-Dimensional Electrophoresis with Silver Staining: A Sensitive Method For Analyzing Complex Protein Mixtures

      • Marcia F. Goldfarb

      Pages 130-133

   7. Brevetoxins and Binding: Sodium Channels Versus Antibodies

      • Daniel G. Baden, Thomas J. Mende, Alina M. Szmant

      Pages 134-143

   8. Preparation of Model Haptens to Express Common Epitopes of PSP Toxins

      • Patrick E. Guire, Peter H. Duquette, Richard A. Amos, C. Jerome Behrens, Mark W. Josephson, Ross P. Chambers

      Pages 145-154

   9. Monoclonal Antibodies (Monoabs) to Ciguatoxin and Related Polyethers

      • Y. Hokama, S. A. A. Honda, M. N. Kobayashi, L. K. Nakagawa, L. K. Shirai, J. T. Miyahara

      Pages 155-166

   10. Immunofluorescent Reagents: Preparation and Analysis

       • John F. Daley

       Pages 167-183

   11. Flow Cytometry and Immunofluorescence in Aquatic Sciences

       • Terry L. Cucci, David Robins

       Pages 184-193

   12. Detection Schemes for Antigen-Antibody Reactions

       • Diether J. Recktenwald, Chia-Huei Chen Chen, Laura Chiu, Morgan Conrad

       Pages 194-206

5. Applications

   1. Front Matter

      Pages 207-207

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   2. Identification of Marine Chroococcoid Cyanobacteria by Immunofluorescence

      • Lisa Campbell

      Pages 208-229

This volume represents a collection of pioneering papers introducing immunochemical techniques to the aquatic sciences. It is the product of a workshop entitled "Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions" held at the University of Southern Maine, Portland, Oct. 5-7, 1986. Funding from many sources made this workshop possible, and is gratefully acknowledged. The reader will note great variability in both the quality and scope of the papers which we have divided 'into four sections: I. Background material on the immune system (borrowed from a recent NIH publication) II. Background material related to aquatic sciences III. Techniques IV. Applications Readers familiar with terminology are directed to start with the original contributions related to aquatic sciences, page 21. Some of the contributions are abstracts only. Others are preliminary, for which we are grateful to the authors for their willingness to share recent sometimes tentative findings with a broader community. Some offer novel and tempting insights, but as they exist, lack adequate standards and controls. Others are thorough and represent major scientific contributions. The promise rests in generating and working with reagents of great specificity enabling orders of magnitude improvements in the sensitivity of our measurements. Authors also address the problems and limitations. We thank the numerous authors and reviewers. In support of every successful endeavor is someone of unusual savvy. For us, this has been David A. Phinney. His leadership, wisdom and wit have been important to the introduction of flow cytometry/sorting and immunochemistry into aquatic research.

• Antigen
• Toxin
• Virus
• bacteria
• ecology
• environment
• enzymes
• infectious
• phytoplankton
• plankton
• plankton ecology
• infectious diseases

• Bigelow Laboratory for Ocean Sciences, W. Boothbay Harbor, USA

  Clarice M. Yentsch, Frances C. Mague

• Dept. of Cell Biology, Smith, Kline and French Laboratory, Swedeland, USA

  Paul K. Horan

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