Scanning Electron Microscopy of Vascular Casts: Methods and Applications

1. Front Matter

   Pages i-xiii

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2. Historical Review and Technical Survey of Vascular Casting and Scanning Electron Microscopy

   • Alois Lametschwandtner, Ursula Lametschwandtner

   Pages 1-11

3. Routine Methods for Vascular Casting and SEM

   • Osamu Ohtani, Takuro Murakami

   Pages 13-25

4. Microvascular Corrosion Casting in Angiogenesis Research

   • Rolf H. Christofferson, B. Ove Nilsson

   Pages 27-37

5. Corrosion Cast Technique Applied to Lymphatic Pathways

   • Anton Castenholz

   Pages 39-51

6. Blood and Lymphatic Microvascular Organization of the Digestive Tract

   • Osamu Ohtani, Takuro Murakami

   Pages 53-69

7. Three-Dimensional Architecture of Blood, Lymphatic, and Biliary Pathways in the Liver by SEM of Corrosion Casts

   • Kazuhide Yamamoto, Tatsuya Itoshima, Takao Tsuji, Takuro Murakami

   Pages 71-82

8. Morphological Features of Absorbing Peripheral Lymphatic Vessels Studied by TEM, SEM, and Three-Dimensional Models

   • Giacomo Azzali

   Pages 83-98

9. Relationship of Blood Microvasculature to Structure and Function in Lymphoid Tissue

   • Aiji Ohtsuka, Robert L. Owen, Takuro Murakami

   Pages 99-109

10. Open and Closed Circulation in the Spleen as Evidenced by SEM of Vascular Casts

    • Tsuneo Fujita, Makoto Kashimura, Kazuo Adachi

    Pages 111-122

11. The Lung Microstructure

    • Dean E. Schraufnagel

    Pages 123-137

12. Postnatal Development and Growth of the Pulmonary Microvasculature

    • Peter H. Burri

    Pages 139-156

13. Microvasculature of Bone and Bone Marrow

    • Fumihiko Iwaku

    Pages 157-169

14. Pericyte Topography of the Microvasculature of Skeletal Muscle: Correlated Analysis of Corrosion Casts and KOH-Digested Specimens

    • Eugenio Gaudio, Luigi Pannarale, Alberto Caggiati, Andrea Maggioni, Giulio Marinozzi, Pietro M. Motta

    Pages 171-180

15. Blood Vascular Casting in Cardiac and Skeletal Muscle

    • Richard F. Potter, Alan C. Groom

    Pages 181-197

16. Vasculature of the Carotid Body as Observed by Scanning Electron Microscopy of Vascular Casts

    • Takehito Taguchi, Takuro Murakami, Aiji Ohtsuka

    Pages 199-204

17. Organization and Age-Related Changes of the Rat Hypophyseal Blood Vascular Plexuses and Portal Vessels as Observed by the Injection Replica SEM Method

    • Takuro Murakami

    Pages 205-220

18. Blood Vascular Casts of the Thyroid Gland in Normal and Experimental Conditions

    • Hisao Fujita, Masato Imada

    Pages 221-229

19. Blood Vascular Beds of Rabbit Uterus and Oviduct: A Study of Corrosion Casts by Scanning Electron Microscopy

    • Wolfgang Kühnel, Lüder C. Busch

    Pages 231-243

20. The Ovary: Three-Dimensional Morphodynamics of the Luteo-Follicular Complex by SEM of Vascular Corrosion Casts and Other EM Techniques

    • Guido Macchiarelli, Stefania A. Nottola, Akio Kikuta, Osamu Ohtani, Takuro Murakami, Pietro M. Motta

    Pages 245-259

Recently, attention has been called to the role that microvascular organization plays in the functional morphology of all organs and tissues, both in normal and pathological conditions.
Since its development by Murakami, the corrosion cast method for scanning electron microscopy has come to be considered one of the most efficient means in clarifying the three-dimensional features of the microcirculation of organs and tissues. Scanning Electron Microscopy of Vascular Casts: Methods and Applications was planned to supply fundamental and new information regarding microcirculation studies to general biologists, anatomists, pathologists and clinicians.
The contributions to this volume, contain original findings and excellent electron micrographs obtained by using recently improved corrosion cast methods.
The rich variety of papers in this book will be useful to many, and will provide both the basic and clinically oriented readers with good ideas, suggestions, and original and worthwhile information.

• Uterus
• bone
• electron microscopy
• lung
• microscopy
• placenta
• tissue

• Department of Human Anatomy, Faculty of Medicine, University of Rome “La Sapienza”, Rome, Italy

  Pietro M. Motta

• Department of Anatomy, Okayama University School of Medicine, Okayama, Japan

  Takuro Murakami

• Department of Anatomy, Osaka University Medical School, Osaka, Japan

  Hisao Fujita

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