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Brown Adipose Tissue

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. David A. Guertin

    1. Program in Molecular Medicine, UMass Chan Medical School, Worcester, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Christian Wolfrum

    1. ETH Zürich, Schwerzenbach, Switzerland

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2448)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Brown Adipose Tissue: A Short Historical Perspective

    • Paul Trayhurn
    Pages 1-18

  3. Brown Fat Anatomy in Humans and Rodents

    • Georgia Colleluori, Jessica Perugini, Angelica Di Vincenzo, Martina Senzacqua, Antonio Giordano, Saverio Cinti
    Pages 19-42

  4. Analysis of Thermogenesis Experiments with CalR

    • Marissa D. Cortopassi, Deepti Ramachandran, William B. Rubio, Daniel Hochbaum, Bernardo L. Sabatini, Alexander S. Banks
    Pages 43-72

  5. A Clearing Method for Three-Dimensional Imaging of Adipose Tissue

    • Zeran Lin, Jingyi Chi, Paul Cohen
    Pages 73-82

  6. Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron

    • Kong Y. Chen, Robert J. Brychta, Nikita S. Israni, Alex Jiang, Hannah J. Lea, Taylor N. Lentz et al.
    Pages 83-96

  7. Fluorescent and Luminescent Methods to Detect Lipolysis

    • Emilio P. Mottillo, James G. Granneman
    Pages 97-106

  8. Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice

    • Markus Heine, Carlotta Corban, Joerg Heeren
    Pages 107-118

  9. Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes

    • Su Myung Jung, Johnny Le, Will G. Doxsey, John A. Haley, Grace Park, David A. Guertin et al.
    Pages 119-130

  10. Activation of UCP1-Independent Ca2+ Cycling Thermogenesis by Wireless Optogenetics

    • Kenji Ikeda, Kazuki Tajima, Yuji Tanabe, Ada S. Y. Poon, Shingo Kajimura
    Pages 131-139

  11. Measurement of Futile Creatine Cycling Using Respirometry

    • Janane F. Rahbani, Edward T. Chouchani, Bruce M. Spiegelman, Lawrence Kazak
    Pages 141-153

  12. Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes

    • Anne Loft, Maja Worm Andersen, Jesper Grud Skat Madsen, Susanne Mandrup
    Pages 155-175

  13. Diet-Induced Thermogenesis: Principles and Pitfalls

    • Jan Nedergaard, Barbara Cannon
    Pages 177-202Open Access

  14. Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice

    • Rajib Mukherjee, Joan Sanchez-Gurmaches
    Pages 203-215

  15. Isolation and Characterization of Human Brown Adipocytes

    • Camilla Scheele, Tora Ida Henriksen, Søren Nielsen
    Pages 217-234

  16. Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The “Goldilocks Solution”

    • Min Li, John R. Speakman
    Pages 235-250

  17. Signaling Lipidomic Analysis of Thermogenic Adipocytes

    • Sean D. Kodani, Valerie Bussberg, Niven R. Narain, Michael A. Kiebish, Yu-Hua Tseng
    Pages 251-271

  18. Blood Sampling for Arteriovenous Difference Measurements Across Interscapular Brown Adipose Tissue in Rat

    • Alberto Mestres-Arenas, Montserrat Cairó, Marion Peyrou, Francesc Villarroya
    Pages 273-282

  19. In Vivo Imaging of Brown Adipose Tissue in Humans: FDG-PET/CT and Beyond

    • Kirsi A. Virtanen
    Pages 283-289

  20. Analysis of Single-Cell/Nucleus Transcriptome Data in Adipose Tissue

    • Wenfei Sun
    Pages 291-306
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Keywords

About this book

This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and 'omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Brown Adipose Tissue: Methods and Protocols provides perspectives and detailed protocols for the benefit of both new BAT researchers looking for guidance as well as seasoned researchers who would liketo expand their toolkits. 


Chapter 12 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Program in Molecular Medicine, UMass Chan Medical School, Worcester, USA

    David A. Guertin

  • ETH Zürich, Schwerzenbach, Switzerland

    Christian Wolfrum

Bibliographic Information

  • Book Title: Brown Adipose Tissue

  • Book Subtitle: Methods and Protocols

  • Editors: David A. Guertin, Christian Wolfrum

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2087-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2086-1Published: 16 February 2022

  • Softcover ISBN: 978-1-0716-2089-2Published: 16 February 2022

  • eBook ISBN: 978-1-0716-2087-8Published: 15 February 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 309

  • Number of Illustrations: 8 b/w illustrations, 64 illustrations in colour

  • Topics: Chemistry/Food Science, general, Physiology, Cell Biology, Cell Biology

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