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Autophagy and Cancer

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Helin Norberg

    1. Karolinska Institute, Stockholm, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Erik Norberg

    1. Karolinska Institute, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2445)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Overview of the Field

    1. Front Matter

      Pages 1-1
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    2. A Balance Between Autophagy and Other Cell Death Modalities in Cancer

      • Anna S. Gorbunova, Gelina S. Kopeina, Boris Zhivotovsky
      Pages 3-24

  3. Chaperone-Mediated Autophagy (CMA)

    1. Front Matter

      Pages 25-25
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    2. Isolation of Autophagy Competent Lysosomes from Cancer Cells by Differential Large-Scale Multilayered Density Gradient Centrifugations

      • Merve Kacal, Helin Vakifahmetoglu-Norberg
      Pages 27-38

    3. CRISPR-Cas9 Gene Editing to Generate Isoform-Specific LAMP-2A Knockout in Human Cancer Cells

      • Ting-ting Yu, Xun Zhou, Helin Vakifahmetoglu-Norberg
      Pages 39-50

  4. Macroautophagy

    1. Front Matter

      Pages 51-51
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    2. Modified LC3 Dot Quantification Method

      • Tahira Anwar, Eeva-Liisa Eskelinen
      Pages 53-64

    3. A Quantitative Flow Cytometry –Based Method for Autophagy Detection Across the Cell Cycle

      • Vitaliy O. Kaminskyy
      Pages 65-74

    4. A Dual HiBiT-GFP-LC3 Lentiviral Reporter for Autophagy Flux Assessment

      • Rainer Will, Katja Bauer, Matthias Kudla, Jetsy Montero-Vergara, Stefan Wiemann, Verena Jendrossek et al.
      Pages 75-98

    5. Measuring Autophagic Cargo Flux with Keima-Based Probes

      • Nikolai Engedal, Tonje Sønstevold, Carsten J. Beese, Sarvini Selladurai, Thea Melcher, Julia E. Simensen et al.
      Pages 99-115

    6. Detection of Nuclear Biomarkers for Chromosomal Instability

      • Carles Pons, Eugenia Almacellas, Albert Tauler, Caroline Mauvezin
      Pages 117-125

    7. Analysis of Autophagic Vesicles in Mitotic Cells

      • Eugenia Almacellas, Marta Garcia-Cajide, Albert Tauler, Caroline Mauvezin
      Pages 127-137

    8. Approaches and Protocols to Analyze Autophagy and Its Role in Death of Apoptosis-Resistant Senescent Tumor Cells

      • Elena Y. Kochetkova, Valery A. Pospelov, Tatiana V. Pospelova
      Pages 139-169

    9. Concepts and Design of Introducing Synthetic MicroRNAs into Mammalian Cells

      • Per Hydbring
      Pages 171-182

    10. Studying Autophagy Using a TMT-Based Quantitative Proteomics Approach

      • Kiran Bala Sharma, Suruchi Aggarwal, Amit Kumar Yadav, Sudhanshu Vrati, Manjula Kalia
      Pages 183-203

  5. Mitophagy

    1. Front Matter

      Pages 205-205
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    2. Immunofluorescence-Based Measurement of Autophagosome Formation During Mitophagy

      • Benjamin S. Padman, Michael Lazarou
      Pages 207-226

    3. Induction and Detection of Mitophagy

      • Maria A. Yapryntseva, Boris Zhivotovsky, Vladimir Gogvadze
      Pages 227-239

  6. Autophagy Analysis In Vivo

    1. Front Matter

      Pages 241-241
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Keywords

About this book

This volume includes comprehensive methods and protocols on autophagic pathways in cancer disorders. Chapters cover basic principles of autophagy methodology in cancer cells, translational approaches, systematic computational analyses of TCGA (The Cancer Genome Atlas) cohorts, CCLE (The Cancer Cell Line Encyclopedia) analysis, CRISPR genetics, metabolomics, biochemistry, and immunohistochemistry methodologies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Autophagy and Cancer: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

Editors and Affiliations

  • Karolinska Institute, Stockholm, Sweden

    Helin Norberg, Erik Norberg

Bibliographic Information

  • Book Title: Autophagy and Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Helin Norberg, Erik Norberg

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2071-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2070-0Published: 26 December 2021

  • Softcover ISBN: 978-1-0716-2073-1Published: 26 December 2022

  • eBook ISBN: 978-1-0716-2071-7Published: 01 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 353

  • Number of Illustrations: 13 b/w illustrations, 84 illustrations in colour

  • Topics: Cancer Research

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