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Angiogenesis

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Andrew V. Benest

    1. Biodiscovery Institute, University of Nottingham, Nottingham, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2441)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Developmental Biology

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo

      • Gabriela D’Amico, Christiana Ruhrberg
      Pages 3-18

    3. A Workflow to Track and Analyze Endothelial Migration During Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy

      • Yan Chen, Paul C. Evans, Robert N. Wilkinson
      Pages 19-28

    4. Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling

      • Lachlan Whitehead, Emma C. Watson, Zoe L. Grant
      Pages 29-39

    5. Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of Fourth Pharyngeal Arch Artery Formation

      • Elena Ioannou, Christiana Ruhrberg
      Pages 41-62

    6. Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina

      • Alessandro Fantin
      Pages 63-73

  3. Adult Physiology and Pathology

    1. Front Matter

      Pages 75-75
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    2. Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin

      • Nikita Ved, Jacinta Isabelle Kalisch-Smith
      Pages 77-83

    3. Analyzing Lymphatic Vessel Patterning in Adult Tissue

      • Louise A. Johnson
      Pages 85-94

    4. Transmission Electron Microscopy of Endothelium

      • Zubair Ahmed Nizamundeen, Loubna ElSaboni, Grace Vetch, Jumana Karim, Chris R. Neal, Claire L. Allen et al.
      Pages 95-103

    5. Measurement of Revascularization in the Hind Limb After Experimental Ischemia in Mice

      • Sohni Ria Bhalla, Federica Riu, Maria J. C. Machado, David O. Bates
      Pages 105-113

    6. Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts

      • Peter Baluk, Donald M. McDonald
      Pages 115-134

    7. Noninvasive Measurement of Retinal Microvascular Permeability During Loss of Endothelial Quiescence

      • Naseeb K. Malhi, David O. Bates, Kenton P. Arkill, Claire L. Allen
      Pages 135-156

    8. An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery

      • Dima Majbour, Ariana D. Suarez-Martinez, Nicholas A. Hodges, Arinola O. Lampejo, Banks M. Lomel, Elijah W. Rice et al.
      Pages 157-170

    9. Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart

      • Konstantinos Klaourakis, Paul R. Riley, Joaquim Miguel Vieira
      Pages 171-181

    10. Quantifying Lymphatic Vessel Density in Human Tissue Samples

      • Stewart G. Martin, Emad Rakha, Sarah J. Storr
      Pages 183-189

    11. Quantifying Vascular Remodeling in the Mouse Spinal Cord

      • Marlene E. Da Vitoria Lobo, Lydia Hardowar, Richard P. Hulse
      Pages 191-200

    12. Quantitative Methods to Assess Adipose Vasculature

      • Martina Rudnicki, Alexandra Pislaru, Tara L. Haas
      Pages 201-221

    13. Quantification of Angiogenesis in Laser Choroidal Neovascularization

      • Kathryn R. Green, Nicholas Beazley-Long, Amy P. Lynch, Claire L. Allen, David O. Bates, Andrew V. Benest
      Pages 223-231
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Keywords

About this book

This detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Angiogenesis: Methods and Protocols provides a meaningful compendium of practical approaches to angiogenesis and endothelial cell biology research.

Editors and Affiliations

  • Biodiscovery Institute, University of Nottingham, Nottingham, UK

    Andrew V. Benest

Bibliographic Information

  • Book Title: Angiogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew V. Benest

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2059-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2058-8Published: 01 February 2022

  • Softcover ISBN: 978-1-0716-2061-8Published: 02 February 2023

  • eBook ISBN: 978-1-0716-2059-5Published: 31 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 429

  • Number of Illustrations: 14 b/w illustrations, 108 illustrations in colour

  • Topics: Developmental Biology, Physiology

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