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Bacterial Virulence

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Ohad Gal-Mor

    1. The Infectious Diseases Research Laboratory, Sheba Medical Center, Tel-Hashomer, Israel

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2427)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
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  2. Molecular Biology and Bioinformatics Methods

    1. Front Matter

      Pages 1-1
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    2. Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated pheS for Counterselection

      • Shai Ran Sapir, Etai Boichis, Anat A. Herskovits
      Pages 3-10

    3. A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria

      • Daniel Tchelet, Dor Salomon
      Pages 11-24

    4. Predicting Type III Effector Proteins Using the Effectidor Web Server

      • Naama Wagner, Doron Teper, Tal Pupko
      Pages 25-36

    5. Assay for Type III Secretion in Escherichia coli

      • Bosko Mitrovic, Neta Sal-Man
      Pages 37-46

    6. Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica

      • Rivka Shem-Tov, Ohad Gal-Mor
      Pages 47-54

  3. Cell Culture and Organoid Models of Infection

    1. Front Matter

      Pages 55-55
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    2. Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay

      • Sara Vilela Pais, Sibel Westerhausen, Erwin Bohn, Samuel Wagner
      Pages 57-71

    3. Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility

      • Leah Rankine-Wilson, Céline Rens, Henok Asfaw Sahile, Yossef Av-Gay
      Pages 73-82

    4. Bone Marrow–Derived Macrophage (BMDM ) Infection by Listeria monocytogenes

      • Etai Boichis, Shai Ran Sapir, Anat A. Herskovits
      Pages 83-93

    5. Preparation and Inflammasome Activation of Murine Bone Marrow–Derived and Resident Peritoneal Macrophages

      • Izumi Sasaki, Tsuneyasu Kaisho
      Pages 95-104

    6. Flow Cytometry–Based Single Cell Analyses of Bacterial Adaptation to Intracellular Environments

      • Marc Schulte, Michael Hensel
      Pages 105-117

    7. Quantification of Microbial Fluorescent Sensors During Live Intracellular Infections

      • Erez Mills, Erik Petersen
      Pages 119-131

    8. Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single-Cell RNA Sequencing

      • Shelly Hen-Avivi, Roi Avraham
      Pages 133-147

    9. Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers

      • Erin C. Boyle, Eva J. Wunschel, Guntram A. Grassl
      Pages 149-163

  4. In Vivo Models of Infection

    1. Front Matter

      Pages 165-165
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    2. Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce

      • Laura Elpers, Michael Hensel
      Pages 167-175

    3. Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity

      • Ling Ning Lam, Debra N. Brunson, Jessica K. Kajfasz, José A. Lemos
      Pages 177-183

    4. Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis

      • Miriam Ravins, Poornima Ambalavanan, Debabrata Biswas, Rachel Ying Min Tan, Kimberly Xuan Zhen Lim, Yael Kaufman et al.
      Pages 185-200
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Keywords

About this book

This detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and beneficial, Bacterial Virulence: Methods and Protocols serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence.


Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • The Infectious Diseases Research Laboratory, Sheba Medical Center, Tel-Hashomer, Israel

    Ohad Gal-Mor

Bibliographic Information

  • Book Title: Bacterial Virulence

  • Book Subtitle: Methods and Protocols

  • Editors: Ohad Gal-Mor

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1971-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1970-4Published: 27 May 2022

  • Softcover ISBN: 978-1-0716-1973-5Published: 27 May 2023

  • eBook ISBN: 978-1-0716-1971-1Published: 26 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 267

  • Number of Illustrations: 18 b/w illustrations, 53 illustrations in colour

  • Topics: Microbiology, Biomedicine, general, Cell Biology

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