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Medulloblastoma

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Abhinav Dey

    1. Department of Pediatrics, Emory University, Atlanta, USA

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  2. Anshu Malhotra

    1. Department of Pediatrics, Emory University, Atlanta, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Neha Garg

    1. Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2423)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Childhood Medulloblastoma: An Overview

    • Yujin Suk, William D. Gwynne, Ian Burns, Chitra Venugopal, Sheila K. Singh
    Pages 1-12

  3. Molecular Assay-Based Methods

    1. Front Matter

      Pages 13-13
      Download chapter PDF

    2. Real-Time PCR Assays on Formalin-Fixed, Paraffin-Embedded Medulloblastomas

      • Ratika Kunder, Neelam Vishwanath Shirsat
      Pages 15-25

    3. Validation of microRNA Target Genes Using Luciferase Reporter assay and Western Blot Analysis

      • Pooja Panwalkar, Atul Khire, Neelam Shirsat
      Pages 27-38

    4. Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY

      • Tara Dobson, Jyothishmathi Swaminathan
      Pages 39-50

    5. Quantification of microRNAs in Cells and Tissues Using Stem-Loop RT PCR and qPCR

      • Pooja Panwalkar, Atul Khire, Neelam Shirsat
      Pages 51-58

    6. Analysis of Microarray Data from Medulloblastoma Tissue Samples

      • Debojyoti Dhar, Gopala Kallapura
      Pages 59-64

    7. Advancement of Single-Cell Sequencing in Medulloblastoma

      • Deepanshu Verma, Namyashree Nayak, Ashuthosh Singh, Ashutosh Kumar Singh, Neha Garg
      Pages 65-83

  4. Cellular Assays and Inhibitors for Medulloblastoma

    1. Front Matter

      Pages 85-85
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    2. Flow Cytometry Approaches to Obtain Medulloblastoma Stem Cells from Bulk Cultures

      • Praveen Kumar, Neha Garg
      Pages 87-94

    3. Drug Screening Assays on Medulloblastoma Stem Cells Using Compound Libraries

      • Ashutosh Singh, Neha Garg
      Pages 95-101

    4. 3D-on-2D: A Physiologically Relevant and Gel-Free In Vitro Coculture Method to Assay Antimetastatic Agents

      • Abhinav Dey, Samuel Eisenberg, Rayna Birnbaum, David J. Sharp
      Pages 103-107

    5. In Vitro Screening Assay for Activators of T-Cell Migration to Solid Tumors

      • Abhinav Dey, David J. Sharp
      Pages 109-113

    6. Impact of Nano Preparation of Phytoconstituents in Medulloblastoma

      • Priyanka Mishra, Y. B. Tripathi
      Pages 115-122

    7. Challenges and Recent Advances of Novel Chemical Inhibitors in Medulloblastoma Therapy

      • Anand Maurya, Upendra Kumar Patel, Jitendra Kumar Yadav, Virender Pratap Singh, Alka Agarwal
      Pages 123-140

    8. Role of Ayurvedic Plants as Anticancer Agents

      • Nikhil Pandey, Y. B. Tripathi
      Pages 141-150

  5. Animal Models in Medulloblastoma

    1. Front Matter

      Pages 151-151
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    2. In Vivo Tumorigenicity Assays Using Subcutaneous and Orthotopic Xenografts in Immunodeficient Mice

      • Kedar Yogi, Rahul Thorat, Neelam Shirsat
      Pages 153-164

    3. Protocols to Manufacture an Oncolytic Measles Virus–Sensitive Immunocompetent Mouse Model of Medulloblastoma

      • Sangeet Lal, Corey Raffel
      Pages 165-177
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Keywords

About this book

This volume details methods and protocols covering multiple aspects of Medulloblastoma. Divided into four parts, chapters guide readers through nucleic acids detection and analysis, cell-based analysis methodologies, and applications of patient-information on designing better experimental strategies for future drug development efforts in Medulloblastoma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Medulloblastoma: Methods and Protocols aims to deliver a clear-cut and standardized set of protocols to a broad scientific community.


Editors and Affiliations

  • Department of Pediatrics, Emory University, Atlanta, USA

    Abhinav Dey, Anshu Malhotra

  • Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

    Neha Garg

Bibliographic Information

  • Book Title: Medulloblastoma

  • Book Subtitle: Methods and Protocols

  • Editors: Abhinav Dey, Anshu Malhotra, Neha Garg

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1952-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1951-3Published: 04 January 2022

  • Softcover ISBN: 978-1-0716-1954-4Published: 05 January 2023

  • eBook ISBN: 978-1-0716-1952-0Published: 03 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 241

  • Number of Illustrations: 5 b/w illustrations

  • Topics: Cancer Research

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