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Synaptic Vesicles

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2417)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Live-Imaging of Axonal Cargoes in Drosophila Brain Explants Using Confocal Microscopy

    • Caroline Medioni, Anne Ephrussi, Florence Besse
    Pages 19-28
  3. Quantitative Analysis of Presynaptic Vesicle Luminal pH in Cultured Neurons

    • Yoshihiro Egashira, Shutaro Katsurabayashi, Shigeo Takamori
    Pages 45-58
  4. Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis

    • Chandresh R. Gajera, Rosemary Fernandez, Nadia Postupna, Kathleen S. Montine, C. Dirk Keene, Sean C. Bendall et al.
    Pages 69-88
  5. Synaptosomes and Metamodulation of Receptors

    • Anna Pittaluga, Mario Marchi
    Pages 99-111
  6. Isolation of Synaptic Vesicles from Mammalian Brain

    • Marcelo Ganzella, Momchil Ninov, Dietmar Riedel, Reinhard Jahn
    Pages 131-145
  7. Synaptic Vesicle Pool Monitoring with Synapto-pHluorin

    • Marc Dahlmanns, Jana Katharina Dahlmanns
    Pages 181-192
  8. Dynole 34-2 and Acrylo-Dyn 2-30, Novel Dynamin GTPase Chemical Biology Probes

    • Jennifer R. Baker, Nicholas S. O’Brien, Kate L. Prichard, Phillip J. Robinson, Adam McCluskey, Cecilia C. Russell
    Pages 221-238
  9. Synthesis of Phthaladyn-29 and Naphthalimide-10, GTP Site Directed Dynamin GTPase Inhibitors

    • Cecilia C. Russell, Kate L. Prichard, Nicholas S. O’Brien, Adam McCluskey, Phillip J. Robinson, Jennifer R. Baker
    Pages 239-258
  10. Back Matter

    Pages 259-260

About this book

This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  

 

Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Editors and Affiliations

  • Department of Psychiatry and Psychotherapy, Universitätsklinikum Erlangen, Erlangen, Germany

    Jana Dahlmanns

  • Institute for Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany

    Marc Dahlmanns

Bibliographic Information

  • Book Title: Synaptic Vesicles

  • Book Subtitle: Methods and Protocols

  • Editors: Jana Dahlmanns, Marc Dahlmanns

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1916-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1915-5Published: 01 February 2022

  • Softcover ISBN: 978-1-0716-1918-6Published: 02 February 2023

  • eBook ISBN: 978-1-0716-1916-2Published: 31 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 260

  • Number of Illustrations: 28 b/w illustrations, 52 illustrations in colour

  • Topics: Neurosciences

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access