Cancer Biomarkers

1. Front Matter

   Pages i-xi

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2. A Method of Bone-Metastatic Tumor Progression Assessment in Mice Using Longitudinal Radiography

   • Matthew R. Eber, Juan Miguel Jiménez-Andrade, Christopher M. Peters, Yusuke Shiozawa

   Pages 1-6

3. Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice

   • Susy Kim, Gagan Deep

   Pages 7-12

4. Method to Development of PET Radiopharmaceutical for Cancer Imaging

   • Naresh Damuka, Kiran Kumar Solingapuram Sai

   Pages 13-22

5. PET Use in Cancer Diagnosis, Treatment, and Prognosis

   • Naresh Damuka, Meghana Dodda, Kiran Kumar Solingapuram Sai

   Pages 23-35

6. Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor

   • Leslimar Rios-Colon, Susy Kim, Yixin Su, Deepak Kumar, Gagan Deep

   Pages 37-43

7. Zebrafish Xenograft Model to Study Human Cancer

   • Ranganatha R. Somasagara, TinChung Leung

   Pages 45-53

8. Assessing Oligomerization Status of Mitochondrial OXPHOS Complexes Via Blue Native Page

   • Jordan Woytash, Joseph R. Inigo, Dhyan Chandra

   Pages 55-62

9. In Vitro Cell Impedance Assay to Examine Antigen-Specific T-Cell-Mediated Melanoma Cell Killing to Support Cancer Immunotherapy Drug Discovery

   • Elizabeth R. Stirling, David R. Soto-Pantoja

   Pages 63-68

10. Methods to Detect Nitric Oxide and Reactive Nitrogen Species in Biological Sample

    • Sharanjot Kaur, Kunj Bihari Gupta, Sandeep Kumar, Shishir Upadhyay, Anil Kumar Mantha, Monisha Dhiman

    Pages 69-76

11. Study of Rotary Cell Culture System-Induced Microgravity Effects on Cancer Biomarkers

    • Ragini Singh, Rana P. Singh

    Pages 77-96

12. A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons

    • Joshua J. Wheeler, John M. Davis, Santosh K. Mishra

    Pages 97-106

13. Zymography and Reverse Zymography for Testing Proteases and Their Inhibitors

    • Preety Choudhary, Vineet Kumar Mishra, Snehasikta Swarnakar

    Pages 107-120

14. Method of Preparation of Cigarette Smoke Extract to Assess Lung Cancer-Associated Changes in Airway Epithelial Cells

    • Hina Agraval, Jiten R. Sharma, Umesh C. S. Yadav

    Pages 121-132

15. Air–Liquid Interface Culture Model to Study Lung Cancer-Associated Cellular and Molecular Changes

    • Hina Agraval, Jiten R. Sharma, Neeraj Dholia, Umesh C. S. Yadav

    Pages 133-144

16. Co-Immunoprecipitation-Blotting: Analysis of Protein-Protein Interactions

    • Li Tan, Raghunatha R. Yammani

    Pages 145-154

17. Methods to Assess Oxidative DNA Base Damage Repair of Apurinic/Apyrimidinic (AP) Sites Using Radioactive and Nonradioactive Oligonucleotide-Based Assays

    • Kunj Bihari Gupta, Sharanjot Kaur, Monisha Dhiman, Anil Kumar Mantha

    Pages 155-163

18. Determining the Size Distribution and Integrity of Extracellular Vesicles by Dynamic Light Scattering

    • Mohammad Aslam Khan, Shashi Anand, Sachin Kumar Deshmukh, Seema Singh, Ajay Pratap Singh

    Pages 165-175

19. Characterization of Exosomal Surface Proteins by Immunogold Labeling

    • Yixin Su, Ashish Kumar, Gagan Deep

    Pages 177-182

20. Scanning Electron Microscopy of Giant Cells from Giant Cell Tumor of Bone

    • Asit Ranjan Mridha, Subhash Chandra Yadav

    Pages 183-191

This detailed volume explores numerous methods used in basic science laboratories to characterize cancer-related biomarkers, vital for better managing cancer burden, including cancer risk assessment, cancer diagnosis, determining cancer progression, and therapeutic response. From a radiography method to an examination of single-cell RNA-seq and computational analysis tools in cancer research, this book delves into many techniques that could provide valuable molecular information about the tumor and its microenvironment components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cancer Biomarkers: Methods and Protocols offers researchers multiple helpful ways to study cancer-associated molecular biomarkers.

• Precision medicine
• Targeted therapy
• Tumor microenvironment
• Cancer risk assessment
• Cancer diagnosis and progression

• Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, USA

  Gagan Deep

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