Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2408)
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Table of contents (21 protocols)
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Front Matter
About this book
This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.
Editors and Affiliations
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Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, USA
Kirankumar S. Mysore
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National Institute of Plant Genome Research, New Delhi, India
Muthappa Senthil-Kumar
Bibliographic Information
Book Title: Plant Gene Silencing
Book Subtitle: Methods and Protocols
Editors: Kirankumar S. Mysore, Muthappa Senthil-Kumar
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1875-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1874-5Published: 25 March 2022
Softcover ISBN: 978-1-0716-1877-6Published: 26 March 2023
eBook ISBN: 978-1-0716-1875-2Published: 24 March 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 328
Number of Illustrations: 7 b/w illustrations, 49 illustrations in colour