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Craniofacial Development

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Sebastian Dworkin

    1. La Trobe University, Bundoora, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2403)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Using Caenorhabditis elegans as a Model for Mechanistic Insights of Craniofacial Development

    • Michael Gruss, Ann K. Corsi
    Pages 1-18

  3. Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development

    • Nishanthi Mathiyalagan, Sebastian Dworkin
    Pages 19-32

  4. Elucidation of Gene Expression Patterns in the Craniofacial Tissues of Mouse Embryos by Wholemount In Situ Hybridization

    • Joshua B. Studdert, Heidi Bildsoe, V. Pragathi Masamsetti, Patrick P. L. Tam
    Pages 33-42

  5. Visualization of the Cartilage and Bone Elements in the Craniofacial Structures by Alcian Blue and Alizarin Red Staining

    • Joshua B. Studdert, Heidi Bildsoe, V. Pragathi Masamsetti, Patrick P. L. Tam
    Pages 43-50

  6. Methodology for Free-Floating Organ Culture of Mid-gestation Maxillary Primordial Tissue

    • M. E. de Vries, Sebastian Dworkin
    Pages 51-61

  7. Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion

    • Jack D. Morgan, Jeremy B. A. Green
    Pages 63-80

  8. Single Cell RNA-Seq: Cell Isolation and Data Analysis

    • Val Yianni, Paul T. Sharpe
    Pages 81-89

  9. Generating Zebrafish RNA-Less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9

    • Priyanka Kumari, Morgan Sturgeon, Gregory Bonde, Robert A. Cornell
    Pages 91-106

  10. Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures

    • Mustafa Mian, Jenny Tan, Robin Yong, Ruth Williams, Agatha Labrinidis, Peter J. Anderson et al.
    Pages 107-127

  11. Micro-CT–Based Bone Microarchitecture Analysis of the Murine Skull

    • Jenny Tan, Agatha Labrinidis, Ruth Williams, Mustafa Mian, Peter J. Anderson, Sarbin Ranjitkar
    Pages 129-145

  12. Characterization of Mammalian In Vivo Enhancers Using Mouse Transgenesis and CRISPR Genome Editing

    • Marco Osterwalder, Stella Tran, Riana D. Hunter, Eman M. Meky, Kianna von Maydell, Anne N. Harrington et al.
    Pages 147-186

  13. Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton

    • Daniela M. Roth, Lakshmi Puttagunta, Daniel Graf
    Pages 187-200

  14. Engineering Epithelial–Mesenchymal Microtissues to Study Cell–Cell Interactions in Development

    • Jacob I. Reynolds, Ross A. Vitek, Peter G. Geiger, Brian P. Johnson
    Pages 201-213

  15. Ex Vivo Culture of Human Cranial Suture Cells

    • Peter J. Anderson
    Pages 215-222

  16. Scaffolds for Use in Craniofacial Bone Regeneration

    • Katherine R. Hixon, Christopher T. Eberlin, Meghana Pendyala, Angela Alarcon de la Lastra, Scott A. Sell
    Pages 223-234

  17. Mandible Explant Assay for the Analysis of Meckel’s Cartilage Development

    • Sophie Wiszniak, Quenten Schwarz
    Pages 235-247

  18. High-Resolution Histology for Craniofacial Studies on Zebrafish and Other Teleost Models

    • A. Huysseune, M. Soenens, J.-Y. Sire, P. E. Witten
    Pages 249-262

  19. Live Imaging of the Dynamics of Mammalian Neural Crest Cell Migration

    • Emma L. Moore, Paul A. Trainor
    Pages 263-276

  20. Salivary Gland Development in Culture

    • Marcia Gaete, Tathyane H. N. Teshima, Lemonia Chatzeli, Abigail S. Tucker
    Pages 277-294
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Keywords

About this book

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. 

Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. 



Editors and Affiliations

  • La Trobe University, Bundoora, Australia

    Sebastian Dworkin

Bibliographic Information

  • Book Title: Craniofacial Development

  • Book Subtitle: Methods and Protocols

  • Editors: Sebastian Dworkin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1847-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1846-2Published: 16 December 2021

  • Softcover ISBN: 978-1-0716-1849-3Published: 16 December 2021

  • eBook ISBN: 978-1-0716-1847-9Published: 15 December 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 313

  • Number of Illustrations: 21 b/w illustrations, 73 illustrations in colour

  • Topics: Developmental Biology, Cell Biology

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