Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2348)
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Table of contents (24 protocols)
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Front Matter
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LncRNA Classification and Utility in Cancer
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Front Matter
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LncRNA Profiling and Discovery
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Front Matter
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Validation of Identified LncRNAs
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Front Matter
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Functional Studies To Decipher the Role of Identified LncRNAs
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Front Matter
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About this book
This volume presents techniques needed for the study of long non-coding RNAs (lncRNAs) in cancer from their identification to functional characterization. Chapters guide readers through identification of lncRNA expression signatures in cancer tissue or liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or Nanopore Sequencing techniques; validation of lncRNA signatures by Real time PCR, digital PCR or in situ hybridization; and functional analysis by siRNA or CRISPR based methods for lncRNA silencing or overexpression. Lipid based nanoparticles for delivery of siRNAs in vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are also treated in this volume. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and practical, Long Non-Coding RNAs in Cancer aims to provide a collection of laboratory protocols, bioinformatic pipelines, and review chapters to further research in this vital field.
Editors and Affiliations
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Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain
Alfons Navarro
Bibliographic Information
Book Title: Long Non-Coding RNAs in Cancer
Editors: Alfons Navarro
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1581-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1580-5Published: 24 June 2021
Softcover ISBN: 978-1-0716-1583-6Published: 25 June 2022
eBook ISBN: 978-1-0716-1581-2Published: 23 June 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 387
Number of Illustrations: 55 illustrations in colour
Topics: Cancer Research, Biomedicine, general, Human Genetics