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Pluripotent Stem-Cell Derived Cardiomyocytes

  • Book
  • © 2021

Overview

Editors:
  1. Yoshinori Yoshida

    1. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2320)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Making Cardiomyocytes from Pluripotent Stem Cells

      • Peter Karagiannis, Yoshinori Yoshida
      Pages 3-7

  3. Generation of Pluripotent Stem Cell-Derived Cardiomyocytes and Cardiac Tissues

    1. Front Matter

      Pages 9-9
      Download chapter PDF

    2. A Method for Cardiac Differentiation , Purification , and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells

      • Yuika Morita, Shugo Tohyama, Jun Fujita, Keiichi Fukuda
      Pages 11-21

    3. Large-Scale Differentiation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture

      • Nagako Sougawa, Shigeru Miyagawa, Yoshiki Sawa
      Pages 23-27

    4. Efficient Method to Dissociate Induced Pluripotent Stem Cell-Derived Cardiomyocyte Aggregates into Single Cells

      • Emiko Ito, Shigeru Miyagawa, Yoshinori Yoshida, Yoshiki Sawa
      Pages 29-33

    5. Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches

      • Kenji Miki, Hirohide Saito, Yoshinori Yoshida
      Pages 35-51

    6. Fabrication of Cardiac Constructs Using Bio-3D Printer

      • Kenichi Arai, Daiki Murata, Shoko Takao, Koichi Nakayama
      Pages 53-63

    7. Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium

      • Junjun Li, Li Liu, Itsunari Minami, Shigeru Miyagawa, Yoshiki Sawa
      Pages 65-73

    8. Construction of Three-Dimensional Cardiac Tissues Using Layer-by-Layer Method

      • Maki Takeda, Shigeru Miyagawa, Mitsuru Akashi, Yoshiki Sawa
      Pages 75-79

    9. Generation of Cylindrical Engineered Cardiac Tissues from Human iPS Cell-Derived Cardiovascular Cell Lineages

      • Hidetoshi Masumoto
      Pages 81-88

  4. Physiological Measurements Using Pluripotent Stem Cell-Derived Cardiomyocytes

    1. Front Matter

      Pages 89-89
      Download chapter PDF

    2. Protocol for Morphological and Functional Phenotype Analysis of hiPS-Derived Cardiomyocytes

      • Jun Li, Jong-Kook Lee
      Pages 91-100

    3. Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies

      • Tadashi Takaki, Yoshinori Yoshida
      Pages 101-110

    4. Multielectrode Array Assays Using Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

      • Daisuke Yoshinaga, Yimin Wuriyanghai, Takeru Makiyama
      Pages 111-119

    5. Electrophysiological Analysis of hiPSC-Derived Cardiomyocytes Using a Patch-Clamp Technique

      • Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai, Daisuke Yoshinaga, Takeru Makiyama
      Pages 121-133

    6. Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human- Induced Pluripotent Stem Cells

      • Misato Nakanishi-Koakutsu, Tadashi Takaki, Kenji Miki, Yoshinori Yoshida
      Pages 135-149

    7. Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis

      • Yasunari Kanda, Ayano Satsuka, Sayo Hayashi, Mihoko Hagiwara-Nagasawa, Atsushi Sugiyama
      Pages 151-160

    8. Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells

      • Daisuke Sasaki, Katsuhisa Matsuura, Tatsuya Shimizu
      Pages 161-170
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Keywords

About this book

This volume provides methodologies for ES and iPS cell technology on the study of cardiovascular diseases. Chapters guide readers through protocols on cardiomyocyte generation from pluripotent stem cells, physiological measurements, bioinformatic analysis, gene editing technology, and cell transplantation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Pluripotent Stem-Cell Derived Cardiomyocytes aims to help researchers set up experiments using pluripotent stem cell-derived cardiac cells.


Editors and Affiliations

  • Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Yoshinori Yoshida

Bibliographic Information

  • Book Title: Pluripotent Stem-Cell Derived Cardiomyocytes

  • Editors: Yoshinori Yoshida

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1484-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1483-9Published: 25 July 2021

  • Softcover ISBN: 978-1-0716-1486-0Published: 26 July 2022

  • eBook ISBN: 978-1-0716-1484-6Published: 24 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 304

  • Number of Illustrations: 25 b/w illustrations, 73 illustrations in colour

  • Topics: Stem Cells, Biomedicine, general, Internal Medicine

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