Overview
- Editors:
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Tanya Parish
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Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, USA
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Anuradha Kumar
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Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, USA
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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Table of contents (31 protocols)
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- Elizabeth Wallace, Debra Hendrickson, Nicholas Tolli, Carolina Mehaffy, María Peña, Jerry A. Nick et al.
Pages 1-58
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- Heena Jagatia, Daire Cantillon
Pages 59-75
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- Megan Lucas, Joan M. Ryan, Jackson Watkins, Kala Early, Nicole A. Kruh-Garcia, Carolina Mehaffy et al.
Pages 77-107
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- Marie-Antoinette Lanéelle, Lucie Spina, Jérôme Nigou, Anne Lemassu, Mamadou Daffé
Pages 109-150
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- Yunlong Li, Pooja Keshavan, Jamie H. Corro, Ravi K. Koripella, Rajendra K. Agrawal, Anil K. Ojha
Pages 151-166
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- Benjamin K. Johnson, Sean M. Thomas, Andrew J. Olive, Robert B. Abramovitch
Pages 167-182
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- Manon Mottet, Cristina Bosmani, Nabil Hanna, Jahn Nitschke, Louise H. Lefrançois, Thierry Soldati
Pages 183-203
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- Giulia Manina, Neeraj Dhar
Pages 205-229
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- Liliana Rodrigues, José A. Aínsa, Miguel Viveiros
Pages 231-245
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- Savannah E. R. Gibson, James Harrison, Jonathan A. G. Cox
Pages 247-260
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- Sydney L. Solomon, Bryan D. Bryson
Pages 261-271
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- Eachan O. Johnson, Deborah T. Hung
Pages 323-342
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- Andrew I. Wong, Jeremy M. Rock
Pages 343-364
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- David Giacalone, Lu Huang, Shumin Tan
Pages 365-383
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- Nicholas Banahene, Benjamin M. Swarts
Pages 385-398
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- Marco A. Riojas, Andrew M. Frank, Samuel R. Greenfield, Stephen P. King, Conor J. Meehan, Michael Strong et al.
Pages 399-457
About this book
This fully updated edition explores the latest techniques to study the challenging, and at times dangerous, genus of bacteria known as mycobacteria with basic methods that are still required for mycobacteriology along with the newer or improved methods that have been developed. The volume features chapters on the basics of DNA isolation, protein isolation, and lipid isolation, as well as more sophisticated techniques for isolation of ribosomes, and continues with sections involving analyzing subcellular fractions, culture methods, sequencing technology, in vitro models, molecular methods, as well as drug discovery applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Mycobacteria Protocols, Fourth Edition serves as an ideal guide for those starting out with their mycobacteria research and also for those who have worked with it for decades.
