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Glycosaminoglycans

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2303)

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Table of contents (60 protocols)

  1. Front Matter

    Pages i-xxi
  2. GAG Structures

    1. Front Matter

      Pages 1-1
    2. Solution NMR Spectroscopy for Characterizing Protein–Glycosaminoglycan Interactions

      • Prem Raj B. Joseph, Krishna Mohan Sepuru, Krishna Mohan Poluri, Krishna Rajarathnam
      Pages 13-23
    3. Fluorous-Tag-Assisted Synthesis of GAG-Like Oligosaccharides

      • José L. de Paz, Pedro M. Nieto
      Pages 37-47
    4. A Nonradioactive Method to Measure Hyaluronan Synthase Activity

      • Davide Vigetti, Evgenia Karousou, Manuela Viola, Alberto Passi
      Pages 63-70
    5. A Glycoproteomic Approach to Identify Novel Proteoglycans

      • Fredrik Noborn, Mahnaz Nikpour, Andrea Persson, Carina Sihlbom, Jonas Nilsson, Göran Larson
      Pages 71-85
    6. Heparan Sulfate Structure: Methods to Study N-Sulfation and NDST Action

      • Anders Dagälv, Anders Lundequist, Beata Filipek-Górniok, Tabea Dierker, Inger Eriksson, Lena Kjellén
      Pages 139-150
    7. Methods for the Production of Recombinant Heparosan, a Critical Heparin Precursor, from Nonpathogenic E. coli Strains

      • Anindita Roy, Varun Garg, Sai K. Mehta, Alessandro Rossi, Kuberan Balagurunathan
      Pages 151-161
    8. Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans

      • Andrea Persson, Emil Tykesson, Ulf Ellervik, Katrin Mani
      Pages 173-182
    9. Aggrecan: Approaches to Study Biophysical and Biomechanical Properties

      • Hadi Tavakoli Nia, Christine Ortiz, Alan Grodzinsky
      Pages 209-226

About this book

This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \

Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchersincluding graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs. 


Editors and Affiliations

  • Departments of Biology, Bioengineering, and Medicinal Chemistry, University of Utah, Salt Lake City, USA

    Kuberan Balagurunathan

  • Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, USA

    Hiroshi Nakato

  • Department of Medicinal Chemistry and Institute for Structural Biology, Drug Discovery and Development, Virginia Commonwealth University, Richmond, USA

    Umesh Desai

  • Department of Neurobiology & Department of Nutrition & Integrative Physiology, University of Utah, Salt Lake City, USA

    Yukio Saijoh

Bibliographic Information

  • Book Title: Glycosaminoglycans

  • Book Subtitle: Methods and Protocols

  • Editors: Kuberan Balagurunathan, Hiroshi Nakato, Umesh Desai, Yukio Saijoh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1398-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1397-9Published: 10 October 2021

  • Softcover ISBN: 978-1-0716-1400-6Published: 10 October 2022

  • eBook ISBN: 978-1-0716-1398-6Published: 09 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XXI, 829

  • Number of Illustrations: 66 b/w illustrations, 114 illustrations in colour

  • Topics: Biochemistry, general, Chemistry/Food Science, general

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access