Lung Cancer

1. Front Matter

   Pages i-xiv

   PDF

2. Automated TTF-1 Immunohistochemistry Assay for the Differentiation of Lung Adenocarcinoma Versus Lung Squamous Cell Carcinoma

   • Rosa Vélez Cintrón, Andrés J. Martínez, Jo Ann Jusino, María Conte-Miller, Adalberto Mendoza

   Pages 1-12

3. Immunohistochemical Detection of p40 Expression in Lung Cancer Clinical Samples

   • Aruna Nambirajan, Deepali Jain

   Pages 13-21

4. Automated Immunohistochemistry Assay for the Detection of Napsin-A in Formalin-Fixed Paraffin-Embedded Tissues from Lung Tumors

   • Rosa Vélez Cintrón, Jo Ann Jusino, María Conte-Miller, Andrés J. Martínez, Adalberto Mendoza

   Pages 23-33

5. Detection of Programmed Cell Death Ligand 1 Expression in Lung Cancer Clinical Samples by an Automated Immunohistochemistry System

   • Edwin Roger Parra, Sharia Hernández Ruiz

   Pages 35-47

6. Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression

   • Edwin Roger Parra, Sharia Hernández Ruiz

   Pages 49-57

7. Creation of Formalin-Fixed, Paraffin-Embedded 3D Lung Cancer Cellular Spheroids for the Optimization of Immunohistochemistry Staining Procedures

   • Jennifer Cabán-Rivera, Camille Chardón-Colón, Alberto Pedraza-Torres, Yoan E. Rodríguez, Raymond Quiñones-Alvarado, Pedro G. Santiago-Cardona

   Pages 59-73

8. Immunoblot Validation of Phospho-Specific Antibodies Using Lung Cancer Cell Lines

   • Wilfredo M. Pedreira-García, Jaileene Pérez-Morales, Camille Chardón-Colón, Jennifer Cabán-Rivera, Pedro G. Santiago-Cardona

   Pages 75-90

9. Detection of Non-Small Lung Cell Carcinoma-Associated Genetic Alterations Using a NanoString Gene Expression Platform Approach

   • Johan Staaf, Mats Jönsson, Anna F. Karlsson

   Pages 91-107

10. A PCR-Based Approach for Driver Mutation Analysis of EGFR, KRAS, and BRAF Genes in Lung Cancer Tissue Sections

    • Rodrigo de Oliveira Cavagna, Leticia Ferro Leal, Flávia Escremim de Paula, Gustavo Noriz Bernardinelli, Rui Manuel Reis

    Pages 109-126

11. 6-Color Crystal Digital PCR^TM for the High-Plex Detection of EGFR Mutations in Non-Small Cell Lung Cancer

    • Jordan Madic, Cécile Jovelet, Imane Dehri, Allison C. Mallory

    Pages 127-144

12. Detection of MET Exon 14 Skipping Alterations in Lung Cancer Clinical Samples Using a PCR-Based Approach

    • Jane S. Y. Sui, Stephen P. Finn, Steven G. Gray

    Pages 145-155

13. Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples

    • Diane Frankel, Elise Kaspi, Patrice Roll

    Pages 157-164

14. A Method for the Establishment of Human Lung Adenocarcinoma Patient-Derived Xenografts in Mice

    • Joanne Lundy, Brendan J. Jenkins, Mohamed I. Saad

    Pages 165-173

15. A Method for the Establishment and Characterization of Mouse Lung Adenocarcinoma Cell Lines that Mimic Traits of Human Adenocarcinomas

    • Magda Spella, Ioannis Lilis, Georgios T. Stathopoulos

    Pages 175-186

16. Whole Transcriptome Sequencing Analysis of Cancer Stem/Progenitor Cells Obtained from Mouse Lung Adenocarcinomas

    • Ansam Sinjab, Reem Daouk, Wassim Abou-Kheir, Humam Kadara

    Pages 187-198

17. In Vivo Imaging of Orthotopic Lung Cancer Models in Mice

    • Peng Liu, Liwei Zhao, Laura Senovilla, Oliver Kepp, Guido Kroemer

    Pages 199-212

18. An Annexin V-FITC—Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line

    • Robin Kumar, Ankit Saneja, Amulya K. Panda

    Pages 213-223

19. Detection of DNA Adduct Formation in Rat Lungs by a Micro-HPLC/MS/MS Approach

    • Angélica B. Sanchez, Camila C. M. Garcia, Paolo Di Mascio, Marisa H. G. Medeiros

    Pages 225-239

20. A Method for Liposome Co-encapsulation of Phenethyl Isothiocyanate and Cisplatin and Determining Its Toxicity Against Lung and Lung Cancer Cells

    • Mengwei Sun, Anthony J. Di Pasqua

    Pages 241-253

This detailed book serves as a laboratory manual containing vital protocols and in-depth discussion involving commonly used experimental approaches for the characterization of several aspects of lung tumor biology. Beginning with an extensive section on biomarker detection, the volume continues with chapters on the genetic and molecular characterization of lung cancer biological samples as well as protocols for the generation of research tools and pre-clinical lung cancer models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Lung Cancer: Methods and Protocols provides a global perspective of research efforts related to lung cancer, while allowing researchers to experimentally probe the different aspects of lung cancer research, including the experimentally relevant tests used in the establishment of lung cancer diagnosis and prognosis, in their laboratories.

• Lung tumor biology
• Immunohistochemistry
• Targeted therapy
• Immunotherapy
• Tumor genetics
• Animal models

• Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico

  Pedro G. Santiago-Cardona

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