Rice Genome Engineering and Gene Editing

1. Front Matter

   Pages i-xv

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2. Genetic Engineering and Tissue Culture of Rice

   1. Front Matter

      Pages 1-1

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   2. Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY)

      • Leyla T. Hathwaik, James G. Thomson, Roger Thilmony

      Pages 3-17

   3. TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology

      • Qinlong Zhu, Yao-Guang Liu

      Pages 19-35

   4. Morphogenic Regulators and Their Application in Improving Plant Transformation

      • Samson Nalapalli, Meral Tunc-Ozdemir, Yuejin Sun, Sivamani Elumalai, Qiudeng Que

      Pages 37-61

   5. A Rapid Method for Stably Transforming Rice Seeds

      • Sudheer Kumar, Neeti Sanan-Mishra

      Pages 63-68

   6. Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector

      • Shuvobrata Majumder, Karabi Datta, Swapan Kumar Datta

      Pages 69-79

   7. Genotype-Independent Regeneration and Transformation Protocol for Rice Cultivars

      • Tanushri Kaul, Sonia Khan Sony, Nitya Meenakshi Raman, Khaled Fathy Abdel Motelb, Jyotsna Bharti

      Pages 81-91

3. Genome Editing and Targeted Integration in Rice

   1. Front Matter

      Pages 93-93

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   2. Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts

      • Simon Sretenovic, Changtian Pan, Xu Tang, Yong Zhang, Yiping Qi

      Pages 95-113

   3. Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvement

      • Amit Das, Pallavi Ghana, Bhojaraja Rudrappa, Rita Gandhi, Venkata Sresty Tavva, Amitabh Mohanty

      Pages 115-134

   4. Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice

      • My Vo Thi Tra, Xiaojia Yin, Ishita Bajal, Christian Paolo Balahadia, William Paul Quick, Anindya Bandyopadhyay

      Pages 135-143

   5. Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencing

      • Guanqing Liu, Yiping Qi, Tao Zhang

      Pages 145-172

   6. Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Construct

      • Martine Bes, Leo Herbert, Thibault Mounier, Anne-Cécile Meunier, Franz Durandet, Emmanuel Guiderdoni et al.

      Pages 173-191

   7. Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice

      • Xu Tang, Yiping Qi, Yong Zhang

      Pages 193-204

   8. Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9

      • Yesuf Teslim Yimam, Jianping Zhou, Sayed Abdul Akher, Xuelian Zheng, Yiping Qi, Yong Zhang

      Pages 205-219

   9. Rice Haploid Inducer Development by Genome Editing

      • Juntao Liu, Dawei Liang, Li Yao, Ya Zhang, Chunxia Liu, Yubo Liu et al.

      Pages 221-230

   10. FLP-Mediated Site-Specific Gene Integration in Rice

       • Vibha Srivastava

       Pages 231-240

   11. Rice Gene Targeting by Homologous Recombination with Positive-Negative Selection Strategy

       • Rie Terada, Zenpei Shimatani

       Pages 241-257

4. Bioinformatics, Cellular, and Molecular Analysis of Rice

   1. Front Matter

      Pages 259-259

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This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.

• CRISPR gene editing
• Crop plants
• Targeted integration
• Gene stacking
• Rice production
• Transgenic traits
• Bioinformatics

• Synthetic Biology, Biofuel and Genome Editing R&D, Reliance Industries Ltd, Navi Mumbai, India

  Anindya Bandyopadhyay

• Crop Improvement and Genetics Research Unit, USDA-ARS, Albany, USA

  Roger Thilmony

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