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Antibody Arrays

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Kelly C. Whittaker

    1. RayBiotech, Peachtree Corners, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Ruo-Pan Huang

    1. RayBiotech Life, Peachtree Corners, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2237)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Sandwich-Based Antibody Arrays for Protein Detection

    • Ying Qing Mao, Brianne Petritis, Ruo-Pan Huang
    Pages 1-10

  3. Well-Based Antibody Arrays

    • Robert S. Matson
    Pages 11-38

  4. One-Step Antibody Arrays

    • Ying Qing Mao, Lin-Hai Li
    Pages 39-44

  5. Global Detection of Proteins by Label-Based Antibody Array

    • Zhizhou Kuang, Li-Pai Chen, Ruochun Huang, Ruo-Pan Huang
    Pages 45-53

  6. Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen

    • Anastasiia Halushkina, William Buchmann, Nathalie Jarroux, Régis Daniel
    Pages 55-67

  7. Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays

    • Abby Jones, Patricia Czarnecki, Lasangi Dhanapala, James F. Rusling
    Pages 69-82

  8. Cytometry Multiplex Bead Antibody Array

    • Benyue Zhang, Gang Xiao, Ying Qing Mao, Zhiqiang Lv, Ruo-Pan Huang
    Pages 83-92

  9. Antibody Arrays: Barcode Technology

    • Liwei Yang, Jun Wang
    Pages 93-102

  10. Reverse Phase Protein Arrays

    • Justin B. Davis, Sydney Andes, Virginia Espina
    Pages 103-122

  11. Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression

    • Ruochun Huang, Chun-Hui Gao, Wenjuan Wu, Ruo-Pan Huang
    Pages 123-128

  12. Methods for Membranes and Other Absorbent Surfaces

    • Jarad J. Wilson, Yun Xi, Ruochun Huang, Ruo-Pan Huang
    Pages 129-139

  13. Precise Chip-to-Chip Reagent Transfer for Cross-Reactivity-Free Multiplex Sandwich Immunoassays

    • François Paquet-Mercier, David Juncker, Sébastien Bergeron
    Pages 141-149

  14. Antibody Printing Technologies

    • Valentin Romanov, Benjamin D. Brooks
    Pages 151-177

  15. Self-Assembling Peptide Hydrogels for 3D Microarrays

    • Greta Bergamaschi, Alessandro Strada, Roberto Frigerio, Marina Cretich, Alessandro Gori
    Pages 179-189

  16. Surface Modification of Glass Slides with Aminosilanes for Microarray Use

    • Chris Stuart
    Pages 191-198

  17. Dried Blood-Based Protein Profiling Using Antibody Arrays

    • Kelly C. Whittaker, Ying Qing Mao, Siwei Zhu, Zhiqiang Lv, Ruo-Pan Huang
    Pages 199-206

  18. Profiling Glycoproteins on Functionalized Reverse Phase Protein Array

    • Ying Zhang, Liyuan Zhang, Anton Iliuk, W. Andy Tao
    Pages 207-215

  19. Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin

    • Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike
    Pages 217-224

  20. Analyzing Signaling Pathways Using Antibody Arrays

    • Hao Tang, Chaohui Duan, Zhizhou Kuang, Ruo-Pan Huang
    Pages 225-236
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Keywords

About this book

This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody arrays pertaining to investigations of specific research topics and biological processes as well as guidance on the methods of processing, analysis, and storage of array data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Antibody Arrays: Methods and Protocols aims to empower the reader with the information required to select the most appropriate array for their research application, with the technical knowledge touse and process the array, and with the knowledge to perform analysis that realizes the maximum benefit from the data generated.

Editors and Affiliations

  • RayBiotech, Peachtree Corners, USA

    Kelly C. Whittaker

  • RayBiotech Life, Peachtree Corners, USA

    Ruo-Pan Huang

Bibliographic Information

  • Book Title: Antibody Arrays

  • Book Subtitle: Methods and Protocols

  • Editors: Kelly C. Whittaker, Ruo-Pan Huang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1064-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1063-3Published: 26 November 2020

  • Softcover ISBN: 978-1-0716-1066-4Published: 27 November 2021

  • eBook ISBN: 978-1-0716-1064-0Published: 25 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 316

  • Number of Illustrations: 21 b/w illustrations, 73 illustrations in colour

  • Topics: Biological Techniques, Chemistry/Food Science, general

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