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Pericytes

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Bruno M. Péault

    1. Orthopedic Hospital Research Center and David Geffen School of Medicine, University of California, Los Angeles, CA, USA, BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2235)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Visualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture

    • Roshini Prakash, Nikita Shivani Thareja, Thomas S. Carmichael, Raymond L. Barnhill, Claire Lugassy, Laurent A. Bentolila
    Pages 1-12

  3. Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu Mice

    • James E. Baily, William C. W. Chen
    Pages 13-26

  4. Assessment of Pericyte Phenotype by Flow Cytometry

    • Nusrat S. Khan, Christopher C. West, Fiona Rossi, Mihaela Crisan
    Pages 27-35

  5. Characterization of Human Pericyte Phenotype by Immunohistochemistry

    • Christopher C. West, Nusrat S. Khan, Mihaela Crisan
    Pages 37-45

  6. A Metabolomics-Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures

    • Enateri V. Alakpa, Christopher C. West, Laura Goldie, Mhairi M. Harper, Karl Burgess, Rein V. Ulijn et al.
    Pages 47-59

  7. Pericyte Ontogeny: The Use of Chimeras to Track a Cell Lineage of Diverse Germ Line Origins

    • Heather C. Etchevers
    Pages 61-87

  8. Imaging Pericytes and the Regulation of Cerebral Blood Flow

    • Katie Boyd, Matthew Hammond-Haley, Rozan Vroman, Catherine N. Hall
    Pages 89-117

  9. Derivation of Pericytes from Human Pluripotent Stem Cells

    • Ayelet Dar, Joseph Itskovitz-Eldor
    Pages 119-125

  10. Assessing the Bone-Forming Potential of Pericytes

    • Carolyn A. Meyers, Chenchao Wang, Jiajia Xu, Hsin Chuan Pan, Jia Shen, Kang Ting et al.
    Pages 127-137

  11. Manipulating Pericyte Function with MicroRNAs

    • Milena Vitiello, Ben Cathcart, Andrea Caporali, Marco Meloni
    Pages 139-153

  12. Isolation of Mammalian Mesoangioblasts: A Subset of Pericytes with Myogenic Potential

    • Giorgia Giacomazzi, Gaia Giovannelli, Alessio Rotini, Domiziana Costamagna, Mattia Quattrocelli, Maurilio Sampaolesi
    Pages 155-167

  13. Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes

    • Ania Stefanska, Angela Briski, Nusrat S. Khan, Christopher Kenyon, Bruno M. Péault, John J. Mullins
    Pages 169-180

  14. Metastatic Dissemination Mimicked in a Multicellular Transwell Assay

    • Courtney König, Anja Runge
    Pages 181-190

  15. Back Matter

    Pages 191-192
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Keywords

About this book

This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials ofa cell lineage of increasing interest and therapeutic significance.  



Editors and Affiliations

  • Orthopedic Hospital Research Center and David Geffen School of Medicine, University of California, Los Angeles, CA, USA, BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK

    Bruno M. Péault

Bibliographic Information

  • Book Title: Pericytes

  • Book Subtitle: Methods and Protocols

  • Editors: Bruno M. Péault

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1056-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1055-8Published: 13 February 2021

  • Softcover ISBN: 978-1-0716-1058-9Published: 13 February 2022

  • eBook ISBN: 978-1-0716-1056-5Published: 12 February 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 192

  • Number of Illustrations: 3 b/w illustrations, 26 illustrations in colour

  • Topics: Cell Biology

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