Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2226)
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Table of contents (23 protocols)
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Front Matter
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Molecular Biology Techniques Applied To Ewing Sarcoma
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Front Matter
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Morpho-Molecular Diagnostics in Ewing Sarcoma
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Front Matter
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Cellular Biology and Cell Culture Techniques
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Animal Models in Ewing Sarcoma
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Keywords
About this book
This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing the molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab protocols comprise in vitro techniques including re-expression and functional analyses of target genes together with in vivo tools and models such as patient-derived xenografts (PDX) and orthotopic xenografts for metastasis assessment in Ewing sarcoma. A full section on bioinformatic approaches to Ewing sarcoma covering ChIPseq, epigenetic and systems biology analyses finalizes this volume. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and clearly written, Ewing sarcoma: Methods and Protocols aims to provide methods to further the study of Ewing sarcoma and bring researchers one step forward in the quest to fight this devastating disease.
Editors and Affiliations
Bibliographic Information
Book Title: Ewing Sarcoma
Book Subtitle: Methods and Protocols
Editors: Florencia Cidre-Aranaz, Thomas G. P. Grünewald
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1020-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1019-0Published: 17 December 2020
Softcover ISBN: 978-1-0716-1022-0Published: 18 December 2021
eBook ISBN: 978-1-0716-1020-6Published: 16 December 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 337
Number of Illustrations: 7 b/w illustrations, 50 illustrations in colour
Topics: Cancer Research