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The Complement System

Innovative Diagnostic and Research Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2227)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. The Benefits of Complement Measurements for the Clinical Practice

    • Anne Grunenwald, Lubka T. Roumenina
    Pages 1-20
  3. Method for Depletion of IgG and IgM from Human Serum as Naive Complement Source

    • Seline A. Zwarthoff, Simone Magnoni, Piet C. Aerts, Kok P. M. van Kessel, Suzan H. M. Rooijakkers
    Pages 21-32
  4. Quantification of Complement Proteins with Special Reference to C1q: Multiplex Versus ELISA Versus Rocket Immunoelectrophoresis Versus Nephelometry

    • Kerstin Sandholm, Barbro Persson, Sliva Abdalla, Camilla Mohlin, Bo Nilsson, Kristina N. Ekdahl
    Pages 33-41
  5. C3dg Quantification by PEG Precipitation and or TRIFMA

    • Anne Troldborg, Jens Christian Jensenius
    Pages 43-49
  6. Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay

    • Per H. Nilsson, Kristin Pettersen, Martin Oppermann, Espen W. Skjeflo, Hilde Fure, Dorte Christiansen et al.
    Pages 51-59
  7. Sheep Erythrocyte Preparation for Hemolytic Tests Exploring Complement Functional Activities

    • Melchior Chabannes, Pauline Bordereau, Paula Vieira Martins, Marie-Agnès Dragon-Durey
    Pages 61-67
  8. Hemolytic Tests Exploring Factor H Functional Activities

    • Melchior Chabannes, Shambhuprasad K. Togarsimalemath, Marie-Agnès Dragon-Durey
    Pages 69-81
  9. Functional Hemolytic Test for Complement Alternative Pathway Convertase Activity

    • Marloes A. H. M. Michels, Nicole C. A. J. van de Kar, Elena B. Volokhina, Bert(L) P. W. J. van den Heuvel
    Pages 83-96
  10. Complement C3 Deposition on Endothelial Cells Revealed by Flow Cytometry

    • Idris Boudhabhay, Anne Grunenwald, Lubka T. Roumenina
    Pages 97-105
  11. Anti-C1q Autoantibodies: Standard Quantification and Innovative ELISA

    • Kinga Csorba, Lucia Schirmbeck, Denise Dubler, Marten Trendelenburg
    Pages 107-114
  12. Anti-C1-Inhibitor Autoantibody Detection by ELISA

    • Chiara Suffritti, Sonia Caccia, Silvia Berra, Debora Parolin, Marco Cicardi
    Pages 115-120
  13. Anti-Ficolin-2 and Anti-Ficolin-3 Autoantibody Detection by ELISA

    • Chantal Dumestre-Pérard, Nicole M. Thielens
    Pages 121-132
  14. Detection of Anti-C3b Autoantibodies by ELISA

    • Maria Radanova, Lubka T. Roumenina, Vasil Vasilev
    Pages 133-139
  15. Detection of Complement Factor B Autoantibodies by ELISA

    • Mihály Józsi, Barbara Uzonyi
    Pages 141-145
  16. Detection of C3 Nephritic Factor by Hemolytic Assay

    • Melchior Chabannes, Véronique Frémeaux-Bacchi, Sophie Chauvet
    Pages 147-158
  17. Detection of Genetic Rearrangements in the Regulators of Complement Activation RCA Cluster by High-Throughput Sequencing and MLPA

    • Jesús García-Fernández, Susana Vilches-Arroyo, Leticia Olavarrieta, Julián Pérez-Pérez, Santiago Rodríguez de Córdoba
    Pages 159-178
  18. Complement Detection in Mouse Kidneys by Immunofluorescence

    • Jennifer Laskowski, Joshua M. Thurman
    Pages 179-189
  19. Complement Detection in Human Tumors by Immunohistochemistry and Immunofluorescence

    • Marie V. Daugan, Margot Revel, Laetitia Lacroix, Catherine Sautès-Fridman, Wolf H. Fridman, Lubka T. Roumenina
    Pages 191-203
  20. Analysis of the Ligand Recognition Specificities of Human Ficolins Using Surface Plasmon Resonance

    • Nicole M. Thielens, Evelyne Gout, Monique Lacroix, Jean-Baptiste Reiser, Christine Gaboriaud
    Pages 205-226

About this book

This volume describes a range of methods used in complement laboratories and how to interpret the data. Chapters detail methods for depletion of IgG and IgM, quantification of complement proteins, C3dg quantification, complement C3 deposition on endothelial cells, anti-C1q auto-antibodies, and methods for assessment of interactions of proteins with heme. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and clearly written, The Complement System: Innovative Diagnostic and Research Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, USPC, Université Paris Descartes, Université Paris Diderot, Paris, France

    Lubka T. Roumenina

Bibliographic Information

  • Book Title: The Complement System

  • Book Subtitle: Innovative Diagnostic and Research Protocols

  • Editors: Lubka T. Roumenina

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1016-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1015-2Published: 14 April 2021

  • Softcover ISBN: 978-1-0716-1018-3Published: 15 April 2022

  • eBook ISBN: 978-1-0716-1016-9Published: 13 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 267

  • Number of Illustrations: 14 b/w illustrations, 41 illustrations in colour

  • Topics: Infectious Diseases

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access