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Metabolic Pathway Engineering

  • Book
  • © 2020

Overview

Editors:
  1. Michael E. Himmel

    1. National Renewable Energy Laboratory, Golden, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Yannick J. Bomble

    1. National Renewable Energy Laboratory, Golden, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2096)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview and Future Directions

    • Yannick J. Bomble, Michael E. Himmel
    Pages 1-3

  3. Genetics of Unstudied Thermophiles for Industry

    • Daehwan Chung, Nicholas S. Sarai, Michael E. Himmel, Yannick J. Bomble
    Pages 5-19

  4. Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum

    • Shuen Hon, Liang Tian, Tianyong Zheng, Jingxuan Cui, Lee R. Lynd, Daniel G. Olson
    Pages 21-43

  5. Methods for Metabolic Engineering of a Filamentous Trichoderma reesei

    • Yat-Chen Chou, Arjun Singh, Qi Xu, Michael E. Himmel, Min Zhang
    Pages 45-50

  6. Methods for Algal Protein Isolation and Proteome Analysis

    • Eric P. Knoshaug, Alida T. Gerritsen, Calvin A. Henard, Michael T. Guarnieri
    Pages 51-59

  7. An Improved Leaf Protoplast System for Highly Efficient Transient Expression in Switchgrass (Panicum virgatum L.)

    • Chien-Yuan Lin, Hui Wei, Bryon S. Donohoe, Melvin P. Tucker, Michael E. Himmel
    Pages 61-79

  8. Characterizing Intracellular Proteomes for Microbes: An Experimental Approach Using Label-Free Protein Quantitation

    • Paul E. Abraham, Robert L. Hettich
    Pages 81-87

  9. Bacterial Differential Expression Analysis Methods

    • Sagar Utturkar, Asela Dassanayake, Shilpa Nagaraju, Steven D. Brown
    Pages 89-112

  10. Measuring Biomass-Derived Products in Biological Conversion and Metabolic Process

    • Chang Geun Yoo, Yunqiao Pu, Arthur J. Ragauskas
    Pages 113-124

  11. Crystallography of Metabolic Enzymes

    • Markus Alahuhta, Michael E. Himmel, Yannick J. Bomble, Vladimir V. Lunin
    Pages 125-139

  12. Measuring Metabolic Enzyme Performance

    • Amanda M. Williams-Rhaesa, Michael W. W. Adams
    Pages 141-147

  13. Gene Editing Technologies for Biofuel Production in Thermophilic Microbes

    • Sharon Smolinski, Emily Freed, Carrie Eckert
    Pages 149-163

  14. Software and Methods for Computational Flux Balance Analysis

    • Peter C. St. John, Yannick J. Bomble
    Pages 165-177

  15. Dynamic Flux Analysis: An Experimental Approach of Fluxomics

    • Wei Xiong, Huaiguang Jiang, PinChing Maness
    Pages 179-196

  16. Network Modeling of Complex Data Sets

    • Piet Jones, Deborah Weighill, Manesh Shah, Sharlee Climer, Jeremy Schmutz, Avinash Sreedasyam et al.
    Pages 197-215

  17. Connecting Microbial Genotype with Phenotype in the Omics Era

    • Yongfu Yang, Mengyu Qiu, Qing Yang, Yu Wang, Hui Wei, Shihui Yang
    Pages 217-233

  18. Back Matter

    Pages 235-236
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Keywords

About this book

This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost. 

Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes.

Editors and Affiliations

  • National Renewable Energy Laboratory, Golden, USA

    Michael E. Himmel, Yannick J. Bomble

Bibliographic Information

  • Book Title: Metabolic Pathway Engineering

  • Editors: Michael E. Himmel, Yannick J. Bomble

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0195-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0194-5Published: 28 July 2020

  • Softcover ISBN: 978-1-0716-0197-6Published: 26 August 2021

  • eBook ISBN: 978-1-0716-0195-2Published: 27 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 236

  • Number of Illustrations: 8 b/w illustrations, 48 illustrations in colour

  • Topics: Biomedical Engineering/Biotechnology, Biochemistry, general

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