Ion Mobility-Mass Spectrometry

1. Front Matter

   Pages i-xi

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2. Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules

   • Caleb B. Morris, James C. Poland, Jody C. May, John A. McLean

   Pages 1-31

3. Metabolomics and Lipidomics

   1. Front Matter

      Pages 33-33

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   2. Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics

      • Melanie T. Odenkirk, Erin S. Baker

      Pages 35-54

   3. Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry

      • Rick Reisdorph, Cole Michel, Kevin Quinn, Katrina Doenges, Nichole Reisdorph

      Pages 55-78

   4. Drift-Tube Ion Mobility-Mass Spectrometry for Nontargeted ′Omics

      • Tim J. Causon, Ruwan T. Kurulugama, Stephan Hann

      Pages 79-94

   5. The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines

      • Nicholas B. Vera, Michelle Clasquin, Stephen L. Coy, Paul Vouros

      Pages 95-101

   6. Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics

      • Zeeshan Hamid, Andrea Armirotti

      Pages 103-117

   7. Lipidomics by HILIC-Ion Mobility-Mass Spectrometry

      • Amy Li, Kelly M. Hines, Libin Xu

      Pages 119-132

   8. A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography–Ion Mobility-Mass Spectrometry

      • Laura Righetti, Chiara Dall’Asta

      Pages 133-144

   9. Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS)

      • Amol Kafle, James Glick, Stephen L. Coy, Paul Vouros

      Pages 145-157

4. Proteomics and Glycomics

   1. Front Matter

      Pages 159-159

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   2. An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry

      • Johannes P. C. Vissers, Michael McCullagh

      Pages 161-178

   3. Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics

      • Lauren J. Tomlinson, Claire E. Eyers

      Pages 179-190

   4. Liquid Extraction Surface Analysis (LESA) High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins

      • Rian L. Griffiths, Klaudia I. Kocurek, Helen J. Cooper

      Pages 191-201

   5. Ion Mobility-Mass Spectrometry of Glycoconjugates

      • Weston B. Struwe, David J. Harvey

      Pages 203-219

5. Ambient Ionization and Imaging Ion Mobility-Mass Spectrometry

   1. Front Matter

      Pages 221-221

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   2. Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry

      • Tiina J. Kauppila

      Pages 223-233

   3. Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation

      • Sylwia A. Stopka, Akos Vertes

      Pages 235-244

   4. Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility

      • James I. Langridge, Emmanuelle Claude

      Pages 245-265

This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as anideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

• IM-MS
• Informatics
• Electrophoretic techniques
• Complex biological sample analysis
• Bioinformatics
• Analyte identification
• Metabolomics

• Department of Medicine and Surgery, University of Milano-Bicocca, Vedano al Lambro, Italy

  Giuseppe Paglia

• Georgetown University, Washington, DC, USA

  Giuseppe Astarita

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