Physical Properties of Biological Membranes and Their Functional Implications

1. Front Matter

   Pages i-xi

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2. Introduction

   • Cecilia Hidalgo

   Pages 1-2

3. Structural Studies on Phospholipid Bilayers by X-Ray Fiber Diffraction Methods

   • Mario Suwalsky

   Pages 3-19

4. 2H and 13C NMR Spectroscopy of Lipid Model Membranes

   • Alfred Blume

   Pages 21-70

5. Applications of Calorimetry to Lipid Model Membranes

   • Alfred Blume

   Pages 71-121

6. Molecular Mobility in Membranes

   • D. Marsh

   Pages 123-145

7. The Acetylcholine Receptor and its Membrane Environment

   • F. J. Barrantes

   Pages 147-175

8. Fluorescence Spectroscopy in the Study of Sarcoplasmic Reticulum Calcium-ATPase

   • Sergio Verjovski-Almeida, Eleonora Kurtenbach

   Pages 177-209

9. Lipid—Protein Interactions in the Function of the Na+ and H+ Pumps Role of Sulfatide

   • F. Zambrano, M. Rojas

   Pages 211-232

10. Back Matter

    Pages 233-236

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This book was originated from a series of lectures given in a course on the physical properties of biological membranes and their functional implica­ tions. The course was intended to allow students to get acquainted with the physical techniques used to study biological membranes. The experience was valuable and we feel that a detailed description of the procedures used and of various examples of the results obtained allowed many students to become familiar with a theme that is not often part of regular courses on membrane physiology or biophysics. This book is designed as a tutorial guide for graduate students interested in understanding how physical methods can be utilized to study the proper­ ties of biological membranes. It includes first a detailed description of applications of physical techniques-such as X-ray fiber diffraction methods (Chapter 1), 2H and 13C NMR spectroscopy (Chapter 2), and calorimetry (Chapter 3)-in the study of the properties of lipid model membranes. A description of how to measure molecular mobility in membranes (Chapter 4) follows, and the book concludes with three chapters in which biological membranes are the subject of study. Chapter 5 deals with the acetylcholine receptor and its membrane environment; Chapter 6 discusses how fluorescence techniques can be applied in the study of the calcium ATPase of sarcoplasmic reticulum; and Chapter 7 explains how protein­ lipid interactions modulate the function of the sodium and proton pumps.

• ATP
• ATPase
• Calcium
• Lipid
• X-ray
• fluorescence
• primary structure
• spectroscopy

• Centro de Estudios Científicos de Santiago, Santiago, Chile

  Cecilia Hidalgo

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