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Fungal Diagnostics

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Louise O'Connor

    1. , NCBES, Molecular Diagnostics Research Gr, National University of Ireland, Galway, Dangan, Ireland

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  2. Barry Glynn

    1. , NCBES, Molecular Diagnostics Research Gr, National University of Ireland, Galway, Dangan, Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Describes key factor methods in introducing any new technology into a diagnostic setting
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 968)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview of Invasive Fungal Infections

    • Nina L. Tuite, Katrina Lacey
    Pages 1-23

  3. Fungal Diagnostics: Review of Commercially Available Methods

    • Javier Yugueros Marcos, David H. Pincus
    Pages 25-54

  4. Rapid Methods for the Extraction and Archiving of Molecular Grade Fungal Genomic DNA

    • Andrew M. Borman, Michael Palmer, Elizabeth M. Johnson
    Pages 55-62

  5. APEX DNA Microarray for the Identification of Pathogenic Fungi

    • Arianna Tavanti, Stefano Landi, Sonia Senesi
    Pages 63-70

  6. Microscopic Detection of Yeasts Using Fluorescence In Situ Hybridization

    • João Inácio, Maria da Luz Martins
    Pages 71-82

  7. Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification

    • Yanan Zhao, David S. Perlin
    Pages 83-92

  8. Differentiation of Fungi Using Hybridization Probes on the LightCycler®

    • Stephan Fricke, Nadja Hilger, Christopher Oelkrug, Arne C. Rodloff, Christian Fricke
    Pages 93-104

  9. Quantitative and Multiplex Detection of Pathogenic Fungi Using Padlock Probes, Generic qPCR, and Suspension Array Readout

    • Magnus Jobs, Ronnie Eriksson, Jonas Blomberg
    Pages 105-118

  10. Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex® xMAP Technology

    • S. Preuner, T. Lion
    Pages 119-139

  11. Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization

    • Yoichiro Okubo, Minoru Shinozaki, Megumi Wakayama, Haruo Nakayama, Daisuke Sasai, Takao Ishiwatari et al.
    Pages 141-147

  12. Application of Chip-Based Flow Cytometry for Amphotericin B and Fluconazole Susceptibility Testing on Candida Strains

    • Orsolya Bouquet, Béla Kocsis, Ferenc Kilár, Ildikó Kustos
    Pages 149-154

  13. Surface Plasmon Resonance Genosensor for the Detection of Fusarium culmorum

    • Michelangelo Pascale, Francesco Zezza, Giancarlo Perrone
    Pages 155-165

  14. Hyperbranching Rolling Circle Amplification, An Improved Protocol for Discriminating Between Closely Related Fungal Species

    • Jiufeng Sun, Sybren de Hoog
    Pages 167-175

  15. Loop-Mediated Isothermal Amplification-Based Detection of Fusarium graminearum

    • Ludwig Niessen
    Pages 177-193

  16. Multiplex-Tandem PCR for Fungal Diagnostics

    • Anna Lau, Keith Stanley, Tania Sorrell
    Pages 195-201

  17. Specific Detection of Pneumocystis jirovecii in Clinical Samples by Flow Cytometry

    • Joana Barbosa, Sofia Costa-de-Oliveira, Ana Teresa Silva, Acácio Gonçalves Rodrigues, Cidália Pina-Vaz
    Pages 203-211

  18. Back Matter

    Pages 213-215
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Keywords

About this book

Invasive fungal infections are a significant cause of morbidity and mortality. Over the past decade there has been a concerted effort to develop reliable methods for the detection of such infections. In Fungal Diagnostics: Methods and Protocols, expert researchers in the field detail the introduction of new technology into a diagnostic setting include ease of use, and rapid turnaround time without compromising sensitivity and specificity. Focusing specifically on fungal detection in clinical settings, fungal diagnostics including, environmental testing, agriculture and food production and veterinary diagnostics. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Fungal Diagnostics: Methods and Protocols seeks to aid scientists into the further study of diagnostic and detection of fungi.

Editors and Affiliations

  • , NCBES, Molecular Diagnostics Research Gr, National University of Ireland, Galway, Dangan, Ireland

    Louise O'Connor, Barry Glynn

Bibliographic Information

  • Book Title: Fungal Diagnostics

  • Book Subtitle: Methods and Protocols

  • Editors: Louise O'Connor, Barry Glynn

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-257-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-256-8Published: 08 January 2013

  • Softcover ISBN: 978-1-4939-5980-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-257-5Published: 09 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 215

  • Topics: Microbiology

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