Principles of Protein X-ray Crystallography

1. Front Matter

   Pages i-xv

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2. Crystallizing a Protein

   • Jan Drenth

   Pages 1-21

3. X-ray Sources and Detectors

   • Jan Drenth

   Pages 22-49

4. Crystals

   • Jan Drenth

   Pages 50-69

5. The Theory of X-ray Diffraction by a Crystal

   • Jan Drenth

   Pages 70-116

6. Average Reflection Intensity and Distribution of Structure Factor Data

   • Jan Drenth

   Pages 117-124

7. Special Forms of the Structure Factor

   • Jan Drenth

   Pages 125-128

8. The Solution of the Phase Problem by the Isomorphous Replacement Method

   • Jan Drenth

   Pages 129-179

9. Phase Improvement

   • Jan Drenth

   Pages 180-202

10. Anomalous Scattering in the Determination of the Protein Phase Angles and the Absolute Configuration

    • Jan Drenth

    Pages 203-218

11. Molecular Replacement

    • Jan Drenth

    Pages 219-243

12. Direct Methods

    • Jan Drenth

    Pages 244-252

13. Laue Diffraction

    • Jan Drenth

    Pages 253-259

14. Refinement of the Model Structure

    • Jan Drenth

    Pages 260-291

15. The Combination of Phase Information

    • Jan Drenth

    Pages 292-297

16. Checking for Gross Errors and Estimating the Accuracy of the Structural Model

    • Jan Drenth

    Pages 298-310

17. Back Matter

    Pages 311-341

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New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up­ to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro­ ductions to their fields. These should serve the needs of one-semester or one-quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface to the Second Edition Since the publication of the previous edition in 1994, X-ray crystallography of proteins has advanced by improvements in existing techniques and by addition of new techniques.

• X-ray
• X-ray diffraction
• biochemistry
• crystal twinning
• crystallography
• protein
• proteins
• scattering
• thermodynamics

• Laboratory of Biophysical Chemistry, Groningen, The Netherlands

  Jan Drenth

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