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  • Book
  • © 2015

Cervical Cancer

Methods and Protocols

  • Includes cutting-edge methods and protocols for researching cervical cancer and high-risk HPV types
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1249)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
  2. Approaches to the Detection and Analysis of HPV Types

    1. Front Matter

      Pages 1-1
  3. Molecular Pathogenesis of CxCA

    1. Front Matter

      Pages 37-37
    2. Replication of Human Papillomavirus in Culture

      • Eric J. Ryndock, Jennifer Biryukov, Craig Meyers
      Pages 39-52
    3. HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocytes

      • Sarah A. Brendle, Neil D. Christensen
      Pages 53-66
    4. Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes

      • Hsu-Kun Wang, Thomas R. Broker, Louise T. Chow
      Pages 93-109
    5. Retroviral Expression of Human Cystatin Genes in HeLa Cells

      • Crystal M. Diep, Gagandeep Kaur, Daniel Keppler, Athena W. Lin
      Pages 121-131
  4. Immune Responses to HPV Infections

    1. Front Matter

      Pages 133-133
    2. Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro

      • Andrew W. Woodham, Adam B. Raff, Diane M. Da Silva, W. Martin Kast
      Pages 135-149
  5. Identification of Novel Potential Drug Targets

    1. Front Matter

      Pages 151-151
    2. Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells

      • Oscar Peralta-Zaragoza, Faustino De-la-O-Gómez, Jessica Deas, Gloria Fernández-Tilapa, Geny del Socorro Fierros-Zárate, Claudia Gómez-Cerón et al.
      Pages 153-171
    3. Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells

      • Wenyi Gu, Nigel McMillan, Chengzhong Yu
      Pages 173-182
    4. Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays

      • Gina J. Ferris, Lauren B. Shunkwiler, Charles A. Kunos
      Pages 183-191
  6. Approaches to Detecting, Typing, and Staging of CxCA

    1. Front Matter

      Pages 201-201

About this book

Representing the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.

Editors and Affiliations

  • Touro University California, Vallejo, USA

    Daniel Keppler, Athena W. Lin

Bibliographic Information

  • Book Title: Cervical Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Daniel Keppler, Athena W. Lin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2013-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2012-9Published: 28 October 2014

  • Softcover ISBN: 978-1-4939-5285-4Published: 22 September 2016

  • eBook ISBN: 978-1-4939-2013-6Published: 27 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 413

  • Number of Illustrations: 28 b/w illustrations, 42 illustrations in colour

  • Topics: Cancer Research, Pharmacy

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access