Editors:
- Presents an overall picture of the current state of knowledge in Cell Line Development
- Designed to offer a state-of-the-art review with in–depth assessments and perspectives of expression engineering through understanding the molecular and cellular basis of cell culture systems
- The various chapters are describing the necessary methodologies for using cell engineering tools for the improvement of cell culture processes
- Describes the most recent methods used to establish mammalian host cell lines and expression systems, optimisation of selection systems to reduce development times and improvement of glycosylation and secretion
Part of the book series: Cell Engineering (CEEN, volume 6)
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Table of contents (12 chapters)
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Front Matter
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Back Matter
About this book
Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms.
This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
Editors and Affiliations
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Dept. Chemical & Biochemical, University College, Dublin, Belfield, Dublin 4, Ireland
Mohamed Al-Rubeai
Bibliographic Information
Book Title: Cell Line Development
Editors: Mohamed Al-Rubeai
Series Title: Cell Engineering
DOI: https://doi.org/10.1007/978-90-481-2245-5
Publisher: Springer Dordrecht
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer Science+Business Media B.V. 2009
Hardcover ISBN: 978-90-481-2244-8Published: 10 September 2009
Softcover ISBN: 978-94-007-3040-3Published: 14 March 2012
eBook ISBN: 978-90-481-2245-5Published: 11 August 2009
Series ISSN: 1389-6946
Series E-ISSN: 2542-9515
Edition Number: 1
Number of Pages: VIII, 253
Topics: Biomedicine general, Cell Biology, Genetic Engineering, Biochemistry, general