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Cryotechniques in Biological Electron Microscopy

  • Book
  • © 1987

Overview

Editors:
  1. Rudolf Alexander Steinbrecht

    1. Max-Planck-Institut für Verhaltensphysiologie, Seewiesen, Germany

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  2. Karl Zierold

    1. Max-Planck-Institut für Systemphysiologie, Dortmund, Germany

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    You can also search for this editor in PubMed   Google Scholar

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Table of contents (16 chapters)

  1. Front Matter

    Pages I-XVII
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  3. General Methodology

    1. Front Matter

      Pages 85-85
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    2. Cryofixation Without Pretreatment at Ambient Pressure

      • Hellmuth Sitte, Ludwig Edelmann, Klaus Neumann
      Pages 87-113

    3. Cryoelectron Microscopy of Vitrified Specimens

      • Jacques Dubochet, Marc Adrian, Jiin-Ju Chang, Jean Lepault, Alasdair W. McDowall
      Pages 114-131

    4. Cryoultramicrotomy

      • Karl Zierold
      Pages 132-148

    5. Freeze-Substitution and Freeze-Drying

      • Rudolf Alexander Steinbrecht, Martin Müller
      Pages 149-172

  4. Special Aspects

    1. Front Matter

      Pages 173-173
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    2. Theory and Practice of High Pressure Freezing

      • Hans Moor
      Pages 175-191

    3. Freeze-Etching of Dispersions, Emulsions and Macromolecular Solutions of Biological Interest

      • Luis Bachmann
      Pages 192-204

    4. High Resolution Metal Replication of Freeze-Dried Specimens

      • Heinz Gross
      Pages 205-215

    5. Immunogold Labelling of Cryosections and Cryofractures

      • Johannes Boonstra, Pim van Maurik, Arie J. Verkleij
      Pages 216-230

    6. Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistry

      • Peter M. Frederik
      Pages 231-239

    7. Scanning Electron Microscopy and X-Ray Microanalysis of Frozen-Hydrated Bulk Samples

      • Alan T. Marshall
      Pages 240-257

    8. Cryofixation of Dynamic Processes in Cells and Organelles

      • Gerd Knoll, Arie J. Verkleij, Helmut Plattner
      Pages 258-271

    9. Cryofixation of Diffusible Elements in Cells and Tissues for Electron Probe Microanalysis

      • Karl Zierold, Rudolf Alexander Steinbrecht
      Pages 272-282

  5. Appendix

    1. Front Matter

      Pages 283-283
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Keywords

About this book

To preserve tissue by freezing is an ancient concept going back pre­ sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan­ ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro­ molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos­ sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro­ duced a wealth of successful applications.

Editors and Affiliations

  • Max-Planck-Institut für Verhaltensphysiologie, Seewiesen, Germany

    Rudolf Alexander Steinbrecht

  • Max-Planck-Institut für Systemphysiologie, Dortmund, Germany

    Karl Zierold

Bibliographic Information

  • Book Title: Cryotechniques in Biological Electron Microscopy

  • Editors: Rudolf Alexander Steinbrecht, Karl Zierold

  • DOI: https://doi.org/10.1007/978-3-642-72815-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1987

  • Softcover ISBN: 978-3-642-72817-4Published: 06 December 2011

  • eBook ISBN: 978-3-642-72815-0Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XVII, 297

  • Topics: Cell Biology, Biological and Medical Physics, Biophysics, Anatomy

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