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Metabolomics

Methods and Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Wolfram Weckwerth

    1. Department of Metabolic Networks, Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 358)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
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  2. Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry

    1. Front Matter

      Pages 1-1
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    2. Metabolite Profiling in Blood Plasma

      • Oliver Fiehn, Tobias Kind
      Pages 3-17

    3. Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles

      • Alexander Erban, Nicolas Schauer, Alisdair R. Fernie, Joachim Kopka
      Pages 19-38

    4. Metabolomic Profiling of Natural Volatiles

      • Yury M. Tikunov, Francel W. A. Verstappen, Robert D. Hall
      Pages 39-53

  3. Metabolomics: Data Integration and Data Mining

    1. Front Matter

      Pages 17-17
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  4. Metabolomics: Data Integration and Data Mining

    1. Integrative Profiling of Metabolites and Proteins

      • Katja Morgenthal, Stefanie Wienkoop, Florian Wolschin, Wolfram Weckwerth
      Pages 57-75

    2. A Gentle Guide to the Analysis of Metabolomic Data

      • Ralf Steuer, Katja Morgenthal, Wolfram Weckwerth, Joachim Selbig
      Pages 105-126

  5. Metabolomics: Data Integration and Data Mining

    1. Integrating Profiling Data

      • Ewa Urbanczyk-Wochniak, Lothar Willmitzer, Alisdair R. Fernie
      Pages 77-85

    2. Visualization and Analysis of Molecular Data

      • Matthias Scholz, Joachim Selbig
      Pages 87-104

  6. Capillary Electrophoresis Coupled to Mass Spectrometry

    1. Front Matter

      Pages 37-37
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    2. Capillary Electrophoresis-Mass Spectrometry for Metabolomics

      • Tomoyoshi Soga
      Pages 129-137

  7. Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation

    1. Front Matter

      Pages 55-55
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    2. Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics

      • Vladimir V. Tolstikov, Oliver Fiehn, Nobuo Tanaka
      Pages 141-155

  8. Electrochemical Detection of Metabolites

    1. Front Matter

      Pages 75-75
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    2. High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors

      • Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah-Daouk, Wayne R. Matson
      Pages 159-174

  9. Metabolic Fluxes

    1. Front Matter

      Pages 175-175
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    2. Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatography-Mass Spectrometry Data

      • Annik Nanchen, Tobias Fuhrer, Uwe Sauer
      Pages 177-197

    3. Understanding the Roadmap of Metabolism by Pathway Analysis

      • Stefan Schuster, Axel von Kamp, Mikhail Pachkov
      Pages 199-226

  10. NMR Metabolome Analysis

    1. Front Matter

      Pages 103-103
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Keywords

About this book

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Reviews

From the reviews:

"This book forms part of an extensive and successful series from Methods in Molecular Biology … . this book provides a good introduction to metabolomics, with useful protocols and relevant data analysis techniques that can be adapted easily to suit any researcher’s needs." (Elizabeth Want, ChemMedChem, Issue 2, 2007)

Editors and Affiliations

  • Department of Metabolic Networks, Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    Wolfram Weckwerth

Bibliographic Information

  • Book Title: Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Wolfram Weckwerth

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-244-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-561-3Published: 01 September 2006

  • Softcover ISBN: 978-1-61737-643-6Published: 19 November 2010

  • eBook ISBN: 978-1-59745-244-1Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 312

  • Number of Illustrations: 71 b/w illustrations, 8 illustrations in colour

  • Topics: Bioinformatics

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