Skip to main content
  • Book
  • © 2017

Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1507)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Establishment of Time- and Cell-Specific RNAi in Caenorhabditis elegans

    • Masayuki Hamakawa, Takaaki Hirotsu
    Pages 67-79
  3. Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing

    • Bharathi Suresh, Suresh Ramakrishna, Hyongbum Kim
    Pages 81-94
  4. eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response

    • Ji-Ye Yin, Zizheng Dong, Jian-Ting Zhang
    Pages 113-127
  5. Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry

    • Mowei Zhou, Si Wu, David L. Stenoien, Zhaorui Zhang, Lanelle Connolly, Michael Freitag et al.
    Pages 153-168
  6. Unbiased Interrogation of 3D Genome Topology Using Chromosome Conformation Capture Coupled to High-Throughput Sequencing (4C-Seq)

    • Rutger W. W. Brouwer, Mirjam C. G. N. van den Hout, Wilfred F. J. van IJcken, Eric Soler, Ralph Stadhouders
    Pages 199-220
  7. Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells

    • Krishna Mohan Parsi, Erica Hennessy, Nicola Kearns, René Maehr
    Pages 221-233
  8. In Vitro Assay to Study Histone Ubiquitination During Transcriptional Regulation

    • Jogender Tushir-Singh, Sanchita Bhatnagar
    Pages 235-244
  9. Determination of Alternate Splicing Events Using Transcriptome Arrays

    • Rebecca S. DeVaux, Marcy Kuentzel, Jason Herschkowitz, Sridar V. Chittur
    Pages 245-259
  10. Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor Gene

    • Matteo Forloni, Thuy Ho, Lisha Sun, Narendra Wajapeyee
    Pages 261-268

About this book

            This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and tools to navigate protocols on key bioinformatics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Pathology, Yale University School of Medicine, New Haven, USA

    Narendra Wajapeyee, Romi Gupta

Bibliographic Information

  • Book Title: Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

  • Editors: Narendra Wajapeyee, Romi Gupta

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6518-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6516-8Published: 10 November 2016

  • Softcover ISBN: 978-1-4939-8223-3Published: 08 June 2018

  • eBook ISBN: 978-1-4939-6518-2Published: 09 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 278

  • Number of Illustrations: 20 b/w illustrations, 29 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access