Skip to main content
SpringerLink
Log in
Book cover

Plant Proteostasis

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. L. Maria Lois

    1. Development Program, CRAG (CSIC-IRTA-UAB-UB) Edifici CRAGCampus, UAB Bellaterra (Cerdanyola del Vallés), Barcelona, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Rune Matthiesen

    1. Computational and Experimental Biology Group, National Health Institute Doctor Ricardo Jorge, IP, Lisbon, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1450)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Ubiquitin Conjugation and Deconjugation Analysis

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Approaches to Determine Protein Ubiquitination Residue Types

      • Qian Chen, Xiaoyuan Yang, Qi Xie
      Pages 3-10

    3. Immunoprecipitation of Cullin-RING Ligases (CRLs) in Arabidopsis thaliana Seedlings

      • Anna Franciosini, Giovanna Serino
      Pages 11-21

    4. Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors

      • Antje Hellmuth, Luz Irina A. Calderón Villalobos
      Pages 23-34

    5. Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes

      • Kamila Kalinowska, Marie-Kristin Nagel, Erika Isono
      Pages 35-44

    6. Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants

      • Katarzyna Zientara-Rytter, Agnieszka Sirko
      Pages 45-54

    7. Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro

      • Christin Naumann, Augustin C. Mot, Nico Dissmeyer
      Pages 55-83

    8. Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases

      • Maria Klecker, Nico Dissmeyer
      Pages 85-94

  3. Ubiquitin-like Protein Conjugation, Deconjugation, and Cell Imaging Studies

    1. Front Matter

      Pages 95-95
      Download chapter PDF

    2. SUMO Chain Formation by Plant Enzymes

      • Konstantin Tomanov, Ionida Ziba, Andreas Bachmair
      Pages 97-105

    3. Kinetic Analysis of Plant SUMO Conjugation Machinery

      • Laura Castaño-Miquel, L. Maria Lois
      Pages 107-123

    4. Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases

      • Gary Yates, Anjil Srivastava, Beatriz Orosa, Ari Sadanandom
      Pages 125-133

    5. Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging

      • Abraham Mas, Montse Amenós, L. Maria Lois
      Pages 135-150

    6. Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8

      • María Esther Pérez-Pérez, Ascensión Andrés-Garrido, José L. Crespo
      Pages 151-159

    7. Detection of Autophagy in Plants by Fluorescence Microscopy

      • Yunting Pu, Diane C. Bassham
      Pages 161-172

  4. Proteomic Analysis and Other Post-Translational Modification Studies

    1. Front Matter

      Pages 173-173
      Download chapter PDF

    2. Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System

      • Séverine Planchais, Laurent Camborde, Isabelle Jupin
      Pages 175-194

    3. Detection and Quantification of Protein Aggregates in Plants

      • Marc Planas-Marquès, Saul Lema A., Núria S. Coll
      Pages 195-203

    4. Determination of Protein Carbonylation and Proteasome Activity in Seeds

      • Qiong Xia, Hayat El-Maarouf-Bouteau, Christophe Bailly, Patrice Meimoun
      Pages 205-212
Back to top

Keywords

About this book

This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture. 

Editors and Affiliations

  • Development Program, CRAG (CSIC-IRTA-UAB-UB) Edifici CRAGCampus, UAB Bellaterra (Cerdanyola del Vallés), Barcelona, Spain

    L. Maria Lois

  • Computational and Experimental Biology Group, National Health Institute Doctor Ricardo Jorge, IP, Lisbon, Portugal

    Rune Matthiesen

Bibliographic Information

  • Book Title: Plant Proteostasis

  • Book Subtitle: Methods and Protocols

  • Editors: L. Maria Lois, Rune Matthiesen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3759-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3757-8Published: 18 July 2016

  • Softcover ISBN: 978-1-4939-8131-1Published: 31 May 2018

  • eBook ISBN: 978-1-4939-3759-2Published: 17 July 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 307

  • Number of Illustrations: 33 b/w illustrations, 40 illustrations in colour

  • Topics: Plant Sciences

Publish with us

Policies and ethics

Back to top

Search

Navigation