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Chromosomal Mutagenesis

  • Book
  • © 2015

Overview

Editors:
  1. Shondra M. Pruett-Miller

    1. Department of Genetics, Genome Engineering and iPSC Center, Washington University School of Medicine, St. Louis, USA

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  • Includes all new cutting-edge methods and protocols for chromosomal mutagenesis research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1239)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Genome Editing by Targeted Chromosomal Mutagenesis

    • Dana Carroll
    Pages 1-13

  3. piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells

    • Stephen J. Pettitt, E-Pien Tan, Kosuke Yusa
    Pages 15-28

  4. Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy

    • Jonathan M. Geisinger, Michele P. Calos
    Pages 29-38

  5. Therapeutic Genome Mutagenesis Using Synthetic Donor DNA and Triplex-Forming Molecules

    • Faisal Reza, Peter M. Glazer
    Pages 39-73

  6. Genome Engineering Using Adeno-Associated Virus (AAV)

    • Rob Howes, Christine Schofield
    Pages 75-103

  7. Engineering of Customized Meganucleases via In Vitro Compartmentalization and In Cellulo Optimization

    • Ryo Takeuchi, Michael Choi, Barry L. Stoddard
    Pages 105-132

  8. Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform

    • Tomas Cermak, Colby G. Starker, Daniel F. Voytas
    Pages 133-159

  9. Ligation-Independent Cloning (LIC) Assembly of TALEN Genes

    • Jonathan L. Schmid-Burgk, Tobias Schmidt, Veit Hornung
    Pages 161-169

  10. Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications

    • Sandrine Boissel, Andrew M. Scharenberg
    Pages 171-196

  11. Genome Engineering Using CRISPR-Cas9 System

    • Le Cong, Feng Zhang
    Pages 197-217

  12. Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases

    • Shondra M. Pruett-Miller, Gregory D. Davis
    Pages 219-229

  13. Endogenous Gene Tagging with Fluorescent Proteins

    • John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, Dmitry Malkov
    Pages 231-240

  14. Silencing Long Noncoding RNAs with Genome-Editing Tools

    • Tony Gutschner
    Pages 241-250

  15. Gene Editing Using ssODNs with Engineered Endonucleases

    • Fuqiang Chen, Shondra M. Pruett-Miller, Gregory D. Davis
    Pages 251-265

  16. Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases

    • Kunitoshi Chiba, Dirk Hockemeyer
    Pages 267-280

  17. Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells

    • Matthew Porteus
    Pages 281-289

  18. Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models

    • Victoria M. Bedell, Stephen C. Ekker
    Pages 291-305

  19. Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection

    • Takehito Kaneko, Tomoji Mashimo
    Pages 307-315

  20. Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains

    • Takehito Kaneko
    Pages 317-329
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Keywords

About this book

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.

Editors and Affiliations

  • Department of Genetics, Genome Engineering and iPSC Center, Washington University School of Medicine, St. Louis, USA

    Shondra M. Pruett-Miller

Bibliographic Information

  • Book Title: Chromosomal Mutagenesis

  • Editors: Shondra M. Pruett-Miller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1862-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1861-4Published: 19 November 2014

  • Softcover ISBN: 978-1-4939-5541-1Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1862-1Published: 19 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 333

  • Number of Illustrations: 22 illustrations in colour

  • Topics: Human Genetics, Genetic Engineering

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