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  • © 2014

Yeast Genetics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1205)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. PCR Mutagenesis and Gap Repair in Yeast

    • Mark Weir, Jill B. Keeney
    Pages 29-35
  3. PCR-Mediated Epitope Tagging of Genes in Yeast

    • Radhika Mathur, Peter Kaiser
    Pages 37-44
  4. Manipulating the Yeast Genome: Deletion, Mutation, and Tagging by PCR

    • Jennifer M. Gardner, Sue L. Jaspersen
    Pages 45-78
  5. Single Yeast Cell Imaging

    • Heimo Wolinski, Sepp D. Kohlwein
    Pages 91-109
  6. Synthetic Genetic Array Analysis for Global Mapping of Genetic Networks in Yeast

    • Elena Kuzmin, Sara Sharifpoor, Anastasia Baryshnikova, Michael Costanzo, Chad L. Myers, Brenda J. Andrews et al.
    Pages 143-168
  7. Chemical Genetic and Chemogenomic Analysis in Yeast

    • Namal V. C. Coorey, Liam D. P. Sampson, Jacqueline M. Barber, David S. Bellows
    Pages 169-186
  8. Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays

    • John Rodgers, Jingyu Guo, John L. Hartman IV
    Pages 187-208
  9. Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast

    • Maria D. Mayán, Luis Aragón
    Pages 219-229
  10. Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq

    • Philippe Lefrançois, Jennifer E. G. Gallagher, Michael Snyder
    Pages 231-255
  11. High-Density Tiling Microarray Analysis of the Full Transcriptional Activity of Yeast

    • Lior David, Sandra Clauder-Münster, Lars M. Steinmetz
    Pages 257-273
  12. Analysis of Silencing in Saccharomyces cerevisiae

    • Andrew Miller, Ann L. Kirchmaier
    Pages 275-302
  13. A User’s Guide to the Ribosomal DNA in Saccharomyces cerevisiae

    • Joseph M. Johnson, Jeffrey S. Smith, David A. Schneider
    Pages 303-328
  14. Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication

    • Sandra K. Villwock, Oscar M. Aparicio
    Pages 329-340

About this book

Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.

Editors and Affiliations

  • Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, USA

    Jeffrey S. Smith, Daniel J. Burke

Bibliographic Information

  • Book Title: Yeast Genetics

  • Book Subtitle: Methods and Protocols

  • Editors: Jeffrey S. Smith, Daniel J. Burke

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1363-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1362-6Published: 12 September 2014

  • Softcover ISBN: 978-1-4939-5182-6Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1363-3Published: 11 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 378

  • Number of Illustrations: 42 b/w illustrations, 15 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access