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This book constitutes the thoroughly refereed post-conference proceedings of the 8th International Conference on Learning and Optimization, LION 8, which was held in Gainesville, FL, USA, in February 2014. The 33 contributions presented were carefully reviewed and selected for inclusion in this book. A large variety of topics are covered, such as algorithm configuration; multiobjective optimization; metaheuristics; graphs and networks; logistics and transportation; and biomedical applications.