Overview
- Nominated by National Taiwan University as an outstanding Ph.D. thesis
- Introduces the synthesis of RM2 antigen using the [1+2+3] one-pot sequential strategy
- Presents the preparation of vaccine candidates and the results of vaccination studies
- Includes total synthesis of a-(2 ? 3)/ a-(2 ? 6)-disialyl ganglioside derivatives as cancer-associated antigens
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (4 chapters)
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Front Matter
Keywords
About this book
This thesis focuses on the synthesis and vaccine evaluation of the prostate tumor- associated carbohydrate antigen RM2. The author first presents the use of the [1+2+3] one-pot sequential strategy to successfully synthesise the RM2 antigen and its analogues as single stereoisomers in every glycosylation step, producing good yields and stereoselectivity. He then introduces the conjugation of the synthetic RM2 antigen to the carrier protein CRM197 in an average number of 1–10 to create the prostate cancer vaccine candidate, which is combined with α-galactosylceramide C1, its analogue C34, or Alu. The results of the vaccination studies in mice are also described and indicate that the strongest anti-RM2 antigen titer is exhibited when one molecule of diphtheria toxin (DT) is conjugated with an average of 4.7 molecules of RM2 antigen (DT-RM4.7) and adjuvanted with the glycolipid C34. More importantly, the induced mouse antibodies mediate the effective complement-dependent cytotoxicity (CDC) against the prostate cancer cell line LNCap. The study presented in this thesis is the first ever to successfully synthesize this complex glycan molecule. Owing to the steric hindrance of the adjacent sialyl moiety, the introduction of two sialic acid units to the compact and rigid 3,4 di branched galactoside unit is very challenging and the β-selective and efficient glycosylation of the galactosamine moiety at the 4-position of di branched galactose is also problematic.
Authors and Affiliations
Bibliographic Information
Book Title: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen RM2 from Prostate Cancer
Authors: Hong-Yang Chuang
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-3-662-46848-7
Publisher: Springer Berlin, Heidelberg
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer-Verlag Berlin Heidelberg 2015
Hardcover ISBN: 978-3-662-46847-0Published: 05 May 2015
Softcover ISBN: 978-3-662-52634-7Published: 29 October 2016
eBook ISBN: 978-3-662-46848-7Published: 23 April 2015
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XIII, 108
Number of Illustrations: 41 b/w illustrations, 26 illustrations in colour
Topics: Carbohydrate Chemistry, Vaccine, Immunology, Medicinal Chemistry