Skip to main content
SpringerLink
Log in

Plant Gene Silencing

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Kirankumar S. Mysore

    1. Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Muthappa Senthil-Kumar

    1. National Institute of Plant Genome Research, New Delhi, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1287)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Review Chapters

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Advances in Plant Gene Silencing Methods

      • Prachi Pandey, Muthappa Senthil-Kumar, Kirankumar S. Mysore
      Pages 3-23

    3. Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies

      • Christophe Lacomme
      Pages 25-41

    4. Bioinformatics Tools for Achieving Better Gene Silencing in Plants

      • Firoz Ahmed, Xinbin Dai, Patrick Xuechun Zhao
      Pages 43-60

    5. Profiling of Small RNAs Involved in Plant–Pathogen Interactions

      • Dongdong Niu, Zhaoyun Wang, Shune Wang, Lulu Qiao, Hongwei Zhao
      Pages 61-79

    6. RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants

      • Abdulrazak B. Ibrahim, Francisco J. L. Aragão
      Pages 81-92

  3. Methods Chapter

    1. Front Matter

      Pages 93-93
      Download chapter PDF

    2. Simplifying Transgene Locus Structure Through Cre-lox Recombination

      • Vibha Srivastava, David W. Ow
      Pages 95-103

    3. Transgene-Induced Gene Silencing in Plants

      • Yun Jin, Hui-Shan Guo
      Pages 105-117

    4. Gene Silencing by DNA Interference in Fern Gametophytes

      • Masamitsu Wada, Hidenori Tsuboi
      Pages 119-127

    5. Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector

      • Akira Kanazawa, Megumi Kasai
      Pages 129-137

    6. A Method for Validating MicroRNAs in Plants by miR-RACE

      • Jinggui Fang, Xin Sun
      Pages 139-145

    7. MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants

      • Weiwei Chen, Qi Zhang, Junhua Kong, Feng Hu, Bin Li, Chaoqun Wu et al.
      Pages 147-157

    8. A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant–Rhizobia Interactions

      • Senjuti Sinharoy, Catalina I. Pislariu, Michael K. Udvardi
      Pages 159-178

    9. A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration

      • Vinay Panwar, Brent McCallum, Guus Bakkeren
      Pages 179-189

    10. An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation

      • Tatsuya Kon, Nubuyuki Yoshikawa
      Pages 191-199

    11. Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using Rice tungro bacilliform virus (RTBV) as a Vector

      • Ravi Kant, Shweta Sharma, Indranil Dasgupta
      Pages 201-217

    12. Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton

      • John R. Tuttle, Candace H. Haigler, Dominique (Niki) Robertson
      Pages 219-234

    13. Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection

      • Ana Marcia E. de A. Manhães, Marcos V. V. de Oliveira, Libo Shan
      Pages 235-241
Back to top

Keywords

About this book

This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.

Editors and Affiliations

  • Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, USA

    Kirankumar S. Mysore

  • National Institute of Plant Genome Research, New Delhi, India

    Muthappa Senthil-Kumar

Bibliographic Information

  • Book Title: Plant Gene Silencing

  • Book Subtitle: Methods and Protocols

  • Editors: Kirankumar S. Mysore, Muthappa Senthil-Kumar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2453-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2452-3Published: 05 March 2015

  • Softcover ISBN: 978-1-4939-4847-5Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2453-0Published: 04 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 307

  • Number of Illustrations: 17 b/w illustrations, 17 illustrations in colour

  • Topics: Plant Genetics and Genomics

Publish with us

Policies and ethics

Back to top

Search

Navigation