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Chemistry - Polymer Science | How Free Cationic Polymer Chains Promote Gene Transfection

How Free Cationic Polymer Chains Promote Gene Transfection

Series: Springer Theses

Yanan, Yue

2013, XVIII, 94 p. 45 illus., 38 illus. in color.

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  • Nominated as an outstanding Ph.D. thesis by the Chinese University of Hong Kong
  • Addresses and elucidates a critical but long overlooked question on non-viral gene delivery
  • Greatly deepens our understanding of polymer-mediated gene transfection, representing a paradigm shift
  • Offers new insights into the rational design of next-generation superior polymeric vectors for gene delivery
In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

Content Level » Research

Keywords » Cyclodextrin-Based Polymers - Endocytic Vescicles - Endocytic-Vesicle-to-Endolysosome Pathway - Free Polycationic Chains - Gene Transfection - Gene-Delivery Vectors - Intracellular Trafficking - Polymer-Mediated Gene Transfection - Polyplex - Proton Sponge

Related subjects » Organic Chemistry - Polymer Science

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