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Photophysics of Ionic Biochromophores

  • Book
  • © 2013

Overview

Editors:
  1. Steen Brøndsted Nielsen

    1. Department of Physics and Astronomy, Aarhus University Faculty of Science, Aarhus, Denmark

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  2. Jean Ann Wyer

    1. Department of Physics and Astronomy, Aarhus University Faculty of Science, Aarhus C, Denmark

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides a concise overview of investigations of ionic biochromophores
  • First integrated overview of the photophysics of different classes of biomolecules: from single amino acids over peptides and proteins to DNA
  • Introduces different experimental techniques for spectroscopic investigation of bio chromophore ions
  • Highly interdisciplinary work with relevance to chemistry, physics and biology/life sciences
  • Includes supplementary material: sn.pub/extras

Part of the book series: Physical Chemistry in Action (PCIA)

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Table of contents (11 chapters)

  1. Front Matter

    Pages i-xiii
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  2. General Concepts

    • Steen Brøndsted Nielsen, Jean Ann Wyer
    Pages 1-10

  3. Introduction and New Aspects

    • Steen Brøndsted Nielsen
    Pages 11-19

  4. Experimental Techniques

    • Jean Ann Wyer
    Pages 21-44

  5. Theoretical Methods

    • Marius Wanko, Angel Rubio
    Pages 45-65

  6. Photo-initiated Dynamics and Spectroscopy of the Deprotonated Green Fluorescent Protein Chromophore

    • Anastasia V. Bochenkova, Lars H. Andersen
    Pages 67-103

  7. Fluorescence from Gas-Phase Biomolecular Ions

    • Steen Brøndsted Nielsen
    Pages 105-116

  8. Spectroscopy of Ferric Heme and Protoporphyrin IX Ions In Vacuo

    • Jean Ann Wyer, Steen Brøndsted Nielsen
    Pages 117-139

  9. UV–Visible Absorption Spectroscopy of Protein Ions

    • Rodolphe Antoine, Philippe Dugourd
    Pages 141-153

  10. Excited-State Dynamics of Protonated Aromatic Amino Acids

    • Claude Dedonder, Géraldine Féraud, Christophe Jouvet
    Pages 155-180

  11. UV Photophysics of DNA and RNA Nucleotides In Vacuo: Dissociation Channels, Time Scales, and Electronic Spectra

    • J. Mathias Weber, Jesse Marcum, Steen Brøndsted Nielsen
    Pages 181-207

  12. Action Spectroscopy of Gas-Phase Peptide Ions with Energetic Photons

    • Thomas Schlathölter, Ronnie Hoekstra
    Pages 209-226

  13. Back Matter

    Pages 227-230
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Keywords

About this book

This book provides a concise overview of the photophysics and spectroscopy of bio chromophore ions. The book "Photophysics of Ionic Biochromophores" summarizes important recent advances in the spectroscopy of isolated biomolecular ions in vacuo, which has within the last decade become a highly active research field. Advanced instrumental apparatus and the steady increase in more and more powerful computers have made this development possible, both for experimentalists and theoreticians. Applied techniques described here include absorption and fluorescence spectroscopy, which are excellent indicators of environmental effects and can thus shed light on the intrinsic electronic structures of ions without perturbations from e.g. water molecules, counter ions, nearby charges, and polar amino acid residues. When compared with spectra of the chromophores in their natural environment, such spectra allow to identify possible perturbations. At the same time gas-phase spectra provide important benchmarks for quantum chemistry calculations of electronically excited states.

This volume focuses on biological systems from protein biochromophores, e.g. the protonated Schiff-base retinal responsible for vision, and individual aromatic amino acids to peptides and whole proteins, studied using visible, ultraviolet and vacuum ultraviolet light. Work on DNA nucleotides and strands that are amenable to mass spectrometric studies because of the negatively charged sugarphosphate backbone are also presented. DNA strands represent an example of the interplay between multiple chromophores, which is even harder to model correctly than just single chromophores due to spatially extended excited states and weak coupling terms. The experimental techniques used to measure spectra and commonly used theoretical methods are described with a discussion on limitations and advantages. The volume includes an updated status of the field and interesting future directions such as cold ionspectroscopy.

Editors and Affiliations

  • Department of Physics and Astronomy, Aarhus University Faculty of Science, Aarhus, Denmark

    Steen Brøndsted Nielsen

  • Department of Physics and Astronomy, Aarhus University Faculty of Science, Aarhus C, Denmark

    Jean Ann Wyer

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